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首页> 外文期刊>Fish & Shellfish Immunology >TAK1-binding proteins (TAB1 and TAB2) in grass carp (Ctenopharyngodon idella): Identification, characterization, and expression analysis after infection with Ichthyophthirius multifiliis
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TAK1-binding proteins (TAB1 and TAB2) in grass carp (Ctenopharyngodon idella): Identification, characterization, and expression analysis after infection with Ichthyophthirius multifiliis

机译：TAK1结合蛋白（TAB1和TAB2）在草鱼（Ctenopharyngodon idella）中的鉴定，鉴定和表达分析

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摘要

Transforming growth factor-beta activated kinase-1 (TAK1) is a key regulatory molecule in toll-like receptor (TLR), interleukin-1 (IL-1), and tumor necrosis factor (TNF) signaling pathways. The activation of TAK1 is specifically regulated by two TAK1-binding proteins, TAB1 and TAB2. However, the roles of TAB1 and TAB2 in fish have not been reported to date. In the present study, TAB1 (CiTAB1) and TAB2 (CiTAB2) in grass carp (Ctenopharyngodon idella) were identified and characterized, and their expression profiles were analyzed after fish were infected with the pathogenic ciliate Ichthyophthirius multifiliis. The fulllength CiTAB1 cDNA is 1949 bp long with an open reading frame (ORF) of 1497 bp that encodes a putative protein of 498 amino acids containing a typical PP2Cc domain. The full-length CiTAB2 cDNA is 2967 bp long and contains an ORF of 2178 bp encoding a putative protein of 725 amino acids. Protein structure analysis revealed that CiTAB2 consists of three main structural domains: an N-terminal CUE domain, a coiled-coil domain, and a C-terminal ZnF domain. Multiple sequence alignment showed that CiTAB1 and CiTAB2 share high sequence identity with other known TAB1 and TAB2 proteins, and several conserved phosphorylation sites and an O-GIcNAc site were deduced in CiTABl. Phylogenetic tree analysis demonstrated that CiTAB1 and CiTAB2 have the closest evolutionary relationship with TAB1 and TAB2 of Danio rerio, respectively. CiTAB1 and CiTAB2 were both widely expressed in all examined tissues with the highest levels in the heart and liver, respectively. After infection with L multifiliis, the expressions of CiTAB1 and CiTAB2 were both significantly up-regulated in all tested tissues at most time points, which indicates that these proteins may be involved in the host immune response against I. multifiliis infection.
机译：转化生长因子-β活化的激酶1（TAK1）是收费样受体（TLR），白介素1（IL-1）和肿瘤坏死因子（TNF）信号通路中的关键调节分子。 TAK1的激活受两个TAK1结合蛋白TAB1和TAB2特异性调节。但是，迄今为止尚未报道TAB1和TAB2在鱼类中的作用。在本研究中，鉴定并鉴定了草鱼（Ctenopharyngodon idella）中的TAB1（CiTAB1）和TAB2（CiTAB2），并分析了鱼被致病性纤毛棘轮棘金鱼（Ichthyophthirius multifiliis）感染后的表达谱。全长CiTAB1 cDNA长1949 bp，开放阅读框（ORF）为1497 bp，编码一个包含典型PP2Cc域的498个氨基酸的推定蛋白质。全长CiTAB2 cDNA长2967 bp，包含2178 bp的ORF，编码725个氨基酸的推定蛋白质。蛋白质结构分析表明，CiTAB2由三个主要结构域组成：N末端CUE域，卷曲螺旋域和C末端ZnF域。多重序列比对显示CiTAB1和CiTAB2与其他已知的TAB1和TAB2蛋白具有高度的序列同一性，并且在CiTAB1中推导了几个保守的磷酸化位点和O-GIcNAc位点。系统进化树分析表明，CiTAB1和CiTAB2分别与Danio rerio的TAB1和TAB2具有最紧密的进化关系。 CiTAB1和CiTAB2均在所有检查过的组织中广泛表达，分别在心脏和肝脏中含量最高。感染了多菌L后，在大多数时间点，所有测试组织中CiTAB1和CiTAB2的表达均显着上调，这表明这些蛋白可能参与了针对多菌I.感染的宿主免疫反应。

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