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首页> 外文期刊>Fish & Shellfish Immunology >Effects of pathogenic bacterial challenge after acute sublethal ammonia-N exposure on heat shock protein 70 expression in Botia reevesae
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Effects of pathogenic bacterial challenge after acute sublethal ammonia-N exposure on heat shock protein 70 expression in Botia reevesae

机译：急性亚致死性氨氮暴露后病原菌攻击对里氏杆菌热激蛋白70表达的影响

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摘要

The objective of this study was to investigate the effects of pathogenic bacterial challenge after acute sublethal ammonia-N exposure on heat shock protein 70 expression in Botia reevesae. After ammonia-N exposure at a constant concentration of 7.21 +/- 0.10 mg L-1 for 96 h, B. reevesae was challenged with Aeromonas hydrophila. Quantitative PCR analysis showed predominant and significant expression of HSP70 in liver, gill, skin, spleen and kidney (P < 0.05), with significantly upregulated expression of the mRNA transcript in these tissues after sublethal ammonia-N exposure and A. hydrophila challenge. Furthermore, following A. hydrophila challenge after ammonia-N exposure, HSP70 mRNA expression was significantly upregulated in kidney and gill tissues, although its expression levels were significantly lower than those detected following A. hydrophila challenge or ammonia-N exposure individually. These results indicate that B. reevesae HSP70 is involved in resistance to pathogenic bacteria. It is hypothesized that ammonia-N results in the downregulation of HSP70 mRNA in immune organs after an A. hydrophila challenge, thus lowering their resistance to pathogenic stress. (C) 2013 Published by Elsevier Ltd.
机译：这项研究的目的是调查急性亚致死性氨氮暴露后致病性细菌攻击对里氏杆菌热激蛋白70表达的影响。在以7.21 +/- 0.10 mg L-1的恒定浓度暴露于氨氮96小时后，用嗜水气单胞菌攻击里氏双歧杆菌。定量PCR分析显示，HSP70在肝，腮，皮肤，脾脏和肾脏中占主要且显着表达（P <0.05），并且在亚致死性氨氮暴露和嗜水杆菌攻击后，这些组织中的mRNA转录表达明显上调。此外，在氨氮暴露后嗜水气单胞菌攻击后，肾脏和ill组织中的HSP70 mRNA表达显着上调，尽管其表达水平明显低于在嗜水气杆菌挑战或氨氮暴露后检测到的水平。这些结果表明里弗氏杆菌HSP70参与了对病原菌的抗性。据推测，氨氮可导致亲水性链球菌攻击后免疫器官中HSP70 mRNA的下调，从而降低其对病原体胁迫的抵抗力。 （C）2013由Elsevier Ltd.出版

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