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首页> 外文期刊>Medicinal and Aromatic Plant Science and Biotechnology >Assessment of Genetic Diversity among Withania somnifera Collected from Central India using RAPD and ISSR Analysis
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Assessment of Genetic Diversity among Withania somnifera Collected from Central India using RAPD and ISSR Analysis

机译：利用RAPD和ISSR分析评价印度中部With Withania somnifera的遗传多样性。

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摘要

Withania somnifera is a multipurpose plant of immense therapeutic value and wide geographic distribution exhibiting extensive pheno-typic and chemical variability. Characterization of plants using molecular markers is an ideal approach for improvementand conservation of plant genetic resources. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) molecular fingerprinting markers were employed as genetic markers to assay the genetic relationship of 16 accessions of W. somnifera collected from different locations of India. Randomly selected 25 decamer primers amplified 204 RAPD marker loci out of which 152 bands (74.5%) were polymorphic. Twenty eight ISSR primers were used to generate fingerprints and a total of 186 alleleswere amplified, out of which 151 were polymorphic (81.1%). ISSR markers were more informative than the RAPD markers. Several ISSR markers amplified genotype-specific alleles that can be used for authentication and detection of adulteration in plant material. Similarity matrices were generated from RAPD and ISSR marker data separately using Jaccard's coefficient and dendrograms were constructed based on UPGMA clustering. Genotypes were clustered into two groups and the grouping was similar for both marker systems.
机译：睡茄（Withania somnifera）是具有巨大治疗价值和广泛地理分布的多用途植物，表现出广泛的表型和化学变异性。使用分子标记物表征植物是改善和保护植物遗传资源的理想方法。随机扩增多态性DNA（RAPD）和简单序列间重复（ISSR）分子指纹标记作为遗传标记，用于分析从印度不同地区收集的16种夜蛾的遗传关系。随机选择的25个decamer引物扩增了204个RAPD标记基因座，其中152条带（74.5％）是多态的。使用二十八个ISSR引物生成指纹，并扩增了186个等位基因，其中151个是多态性的（占81.1％）。 ISSR标记比RAPD标记提供更多信息。几种ISSR标记扩增了特定于基因型的等位基因，可用于鉴定和检测植物材料中的掺假。使用Jaccard系数分别从RAPD和ISSR标记数据生成相似性矩阵，并基于UPGMA聚类构建树状图。基因型被分为两组，两种标记系统的分组相似。

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