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首页> 外文期刊>Molecular biotechnology >A Single buffer That Universally Serves Both Restriction digestion and Loading
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A Single buffer That Universally Serves Both Restriction digestion and Loading

机译：通用服务于限制消化和装载的单个缓冲液

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Restriction digestion is routinely performed in a buffer compatible with the restriction enzyme used. To load the samples on agarose gels for electrophoresis it is then necessary to add a loading buffer. A 10X loading buffer is often used, and consists of a dye to track the electrophoresis and a dense solution so that the digestion mixture sinks into the well. We describe anew buffer, which acts as a universal digestion buffer a well as a loading buffer. This avoids double handling of samples, which wastes both time and consumables. Importantly, the efficiency of digestion was found not to be significantly decreased in this new buffer.
机译：限制酶切消化通常在与所用限制酶兼容的缓冲液中进行。要将样品加载到琼脂糖凝胶上进行电泳，则需要添加加载缓冲液。通常使用10X上样缓冲液，该缓冲液由跟踪电泳的染料和浓溶液组成，使消化混合物沉入孔中。我们描述了一个新的缓冲区，它既充当通用消化缓冲区又充当加载缓冲区。这样避免了样品的重复处理，从而浪费了时间和消耗品。重要的是，发现在这种新缓冲液中消化效率没有显着降低。

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