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首页> 外文期刊>Molecular medicine reports >Isolation, in vitro culture and identification of a new type of mesenchymal stem cell derived from fetal bovine lung tissues
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Isolation, in vitro culture and identification of a new type of mesenchymal stem cell derived from fetal bovine lung tissues

机译：胎牛肺组织来源的新型间充质干细胞的分离，体外培养及鉴定

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Lung-derived mesenchymal stem cells (LMSCs) are considered to be important in lung tissue repair and regenerative processes. However, the biological characteristics and differentiation potential of LMSCs remain to be elucidated. In the present study, fetal lung-derived mesenchymal stem cells (FLMSCs) were isolated from fetal bovine lung tissues by collagenase digestion. The in vitro culture conditions were optimized and stabilized and the self-renewal ability and differentiation potential were evaluated. The results demonstrated that the FLMSCs were morphologically consistent with fibroblasts, were able to be cultured and passaged for at least 33 passages and the cell morphology and proliferative ability were stable during the first 10 passages. In addition, FLMSCs were found to express CD29, CD44, CD73 and CD166, however, they did not express hematopoietic cell specific markers, including CD34, CD45 and BOLA-DR alpha. The growth kinetics of FLMSCs consisted of a lag phase, a logarithmic phase and a plateau phase, and as the passages increased, the proliferative ability of cells gradually decreased. The majority of FLMSCs were in G0/G1 phase. Following osteogenic induction, FLMSCs were positive for the expression of osteopontin and collagen type I alpha 2. Following neurogenic differentiation, the cells were morphologically consistent with neuronal cells and positive for microtubule-associated protein 2 and nestin expression. It was concluded that the isolated FLMSCs exhibited typical characteristics of mesenchymal stem cells and that the culture conditions were suitable for their proliferation and the maintenance of stemness. The present study illustrated the potential application of lung tissue as an adult stem cell source for regenerative therapies.
机译：肺源间充质干细胞（LMSC）被认为在肺组织修复和再生过程中很重要。然而，LMSCs的生物学特性和分化潜能仍有待阐明。在本研究中，通过胶原酶消化从胎牛肺组织中分离出了胎肺来源的间充质干细胞（FLMSC）。优化和稳定了体外培养条件，并评估了其自我更新能力和分化潜能。结果表明，FLMSCs在形态学上与成纤维细胞一致，能够被培养和传代至少33代，并且在前10代中细胞形态和增殖能力是稳定的。另外，发现FLMSC表达CD29，CD44，CD73和CD166，但是它们不表达造血细胞特异性标记，包括CD34，CD45和BOLA-DRα。 FLMSCs的生长动力学由滞后期，对数期和平稳期组成，随着传代次数的增加，细胞的增殖能力逐渐降低。大多数FLMSC处于G0 / G1期。成骨诱导后，FLMSCs骨桥蛋白和I型胶原2型胶原表达阳性。神经源性分化后，细胞在形态学上与神经元细胞一致，微管相关蛋白2和巢蛋白表达阳性。结论是分离出的FLMSCs具有间充质干细胞的典型特征，培养条件适合于它们的增殖和维持干性。本研究说明了肺组织作为成人干细胞再生疗法的潜在应用。

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