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首页> 外文期刊>Molecules >In Vitro Antioxidant and Antiproliferative Activities of Methanolic Plant Part Extracts of Theobroma cacao
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In Vitro Antioxidant and Antiproliferative Activities of Methanolic Plant Part Extracts of Theobroma cacao

机译：可可豆甲醇植物部分提取物的体外抗氧化和抗增殖活性

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摘要

The aims of this study were to determine the antioxidant and antiproliferative activity of the following Theobroma cacao plant part methanolic extracts: leaf, bark, husk, fermented and unfermented shell, pith, root, and cherelle. Antioxidant activity was determined using 2,2-diphenyl-2-picrylhydrazyl (DPPH), thiobarbituric acid-reactive substances (TBARS), and Folin-Ciocalteu assays; the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) assay was used to determine antiproliferative activity. The root extract had the highest antioxidant activity; its median effective dose (EC50) was 358.3 +/- 7.0 mu g/mL and total phenolic content was 22.0 +/- 1.1 g GAE/100 g extract as compared to the other methanolic plant part extracts. Only the cherelle extract demonstrated 10.4% +/- 1.1% inhibition activity in the lipid peroxidation assay. The MTT assay revealed that the leaf extract had the highest antiproliferative activity against MCF-7 cells [median inhibitory concentration (IC50) = 41.4 +/- 3.3 mu g/mL]. Given the overall high IC50 for the normal liver cell line WRL-68, this study indicates that T. cacao methanolic extracts have a cytotoxic effect in cancer cells, but not in normal cells. Planned future investigations will involve the purification, identification, determination of the mechanisms of action, and molecular assay of T. cacao plant extracts.
机译：这项研究的目的是确定以下可可可植物部分甲醇提取物的抗氧化和抗增殖活性：叶，树皮，果壳，发酵和未发酵的壳，髓，根和小elle。使用2,2-二苯基-2-吡啶并肼基（DPPH），硫代巴比妥酸反应性物质（TBARS）和Folin-Ciocalteu测定法确定抗氧化活性;使用3- [4,5-二甲基噻唑-2-基] -2,5-二苯基四唑鎓（MTT）测定抗增殖活性。根提取物具有最高的抗氧化活性。与其他甲醇植物部分提取物相比，其平均有效剂量（EC50）为358.3 +/- 7.0μg / mL，总酚含量为22.0 +/- 1.1 g GAE / 100 g提取物。在脂质过氧化测定中，仅小叶提取物显示出10.4％+/- 1.1％的抑制活性。 MTT分析表明，叶提取物对MCF-7细胞具有最高的抗增殖活性[中位抑制浓度（IC50）= 41.4 +/- 3.3μg / mL]。考虑到正常肝细胞系WRL-68的总体IC50较高，这项研究表明可可木霉甲醇提取物在癌细胞中具有细胞毒性作用，而在正常细胞中则没有。计划的未来研究将涉及可可三叶草植物提取物的纯化，鉴定，作用机理的确定和分子测定。

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