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首页> 外文期刊>Mutation Research, C. Mutation Research Letters >EFFECT OF DIMETHYLTHIOUREA ON CHROMIUM (VI)-INDUCED DNA SINGLE-STRAND BREAKS IN CHINESE HAMSTER V-79 CELLS
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EFFECT OF DIMETHYLTHIOUREA ON CHROMIUM (VI)-INDUCED DNA SINGLE-STRAND BREAKS IN CHINESE HAMSTER V-79 CELLS

机译：二甲基硫脲对中国仓鼠V-79细胞中铬（VI）诱导的DNA单链断裂的影响

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摘要

The effect of 1,3-dimethyl-2-thiourea (DMTU), a specific hydroxyl radical scavenger on chromate-induced DNA breaks, was studied using Chinese hamster V-79 cells. Incubation of cells with Na2CrO4 plus DMTU resulted in a small but significant decrease in cellular levels of the metal-caused DNA single-strand breaks. Electron spin resonance studies showed that DMTU did not affect the formation of chromium (V) complexes either in cells or in the reaction of Na2CrO4 with reduced glutathione in vitro, however, DMTU suppressed the generation of hydroxyl radicals in the reaction of hydrogen peroxide and chromium (V) in vitro. Thus, Na2CrO4-induced DNA breaks were inhibited by DMTU, possibly due to its ability to scavenge hydroxyl radicals. These and other previous studies indicated that the formation of hydroxyl radicals contribute to the induction of DNA breaks by Na2CrO4 in intact cells, but presumably is not the only mechanism involved.
机译：使用中国仓鼠V-79细胞研究了一种特定的羟基自由基清除剂1,3-二甲基-2-硫脲（DMTU）对铬酸盐诱导的DNA断裂的影响。用Na2CrO4和DMTU孵育细胞会导致金属引起的DNA单链断裂的细胞水平轻微但显着降低。电子自旋共振研究表明，DMTU不会在细胞中或在Na2CrO4与还原型谷胱甘肽的体外反应中影响铬（V）络合物的形成，但是，DMTU抑制了过氧化氢与铬反应中羟基自由基的产生（五）体外。因此，DMTU可以抑制Na2CrO4诱导的DNA断裂，这可能是由于其清除羟基自由基的能力。这些和其他先前的研究表明，羟基自由基的形成有助于完整细胞中Na2CrO4诱导DNA断裂，但据推测并不是唯一的机制。

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