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首页> 外文期刊>Mutation Research, C. Mutation Research Letters >SNG1 - A NEW GENE INVOLVED IN NITROSOGUANIDINE RESISTANCE IN SACCHAROMYCES CEREVISIAE
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SNG1 - A NEW GENE INVOLVED IN NITROSOGUANIDINE RESISTANCE IN SACCHAROMYCES CEREVISIAE

机译：SNG1-酿酒酵母中亚硝基胍的抗性新基因

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摘要

We have molecularly characterized the SNG1 gene that confers hyper-resistance to the mutagen N-methyl-N'nitro-N-nitrosoguanidine (MNNG) in Saccharomyces cerevisiae when overexpressed on a multi-copy plasmid. This hyper-resistance to MNNG is not due to depletion of glutathione pools since multi-copy SNG1 containing yeast transformants contain at least wild type levels of glutathione; DNA repair seems unaffected in these transformants as the multi-copy SNG1-mediated MNNG hyper-resistance is also seen in DNA repair mutants belonging to each of the three epistasis groups of yeast repair mutants. It could be shown that SNG1 is not under control of the YAP1 encoded transcription activator that controls expression of at least two genes involved in MNNG metabolism in yeast. sng1 null mutants are viable but exhibit only slight sensitivity to MNNG, indicating that SNG1 does not encode a protein involved in a major detoxification step of this mutagen. Sequencing of the HYR-mediating passenger DNA revealed that SNG1 encodes a 547 aa polypeptide containing seven transmembrane-spanning regions that may be membrane-bound. Comparison of the DNA sequence with established gene databanks revealed that SNG1 is a novel yeast gene.
机译：我们已经在分子上表征了SNG1基因，当在多拷贝质粒上过表达时，它赋予酿酒酵母中的诱变N-甲基-N'硝基-N-亚硝基胍（MNNG）高抗性。这种对MNNG的超抗性不是由于谷胱甘肽池的耗尽所致，因为含有多拷贝SNG1的酵母转化体至少含有野生型水平的谷胱甘肽; DNA修复似乎在这些转化体中不受影响，因为在属于酵母修复突变体三个上位基因组的DNA修复突变体中也发现了多拷贝SNG1介导的MNNG高抗性。可以证明，SNG1不受YAP1编码的转录激活子的控制，该转录激活子控制着酵母中至少两个参与MNNG代谢的基因的表达。 sng1无效突变体是可行的，但对MNNG仅表现出轻微的敏感性，这表明SNG1不编码参与该诱变剂主要排毒步骤的蛋白质。 HYR介导的客运DNA的测序表明SNG1编码547个aa多肽，其中包含七个跨膜区域，这些区域可能是膜结合的。 DNA序列与已建立的基因数据库的比较表明SNG1是一个新的酵母基因。

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