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首页> 外文期刊>Mutation Research, C. Mutation Research Letters >THE SUITABILITY OF RAT PERIPHERAL BLOOD IN SUBCHRONIC STUDIES FOR THE MICRONUCLEUS ASSAY
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THE SUITABILITY OF RAT PERIPHERAL BLOOD IN SUBCHRONIC STUDIES FOR THE MICRONUCLEUS ASSAY

机译：在亚慢性研究中微核试验对大鼠外周血的适应性

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摘要

To examine the suitability of using rat peripheral blood from animals used in subchronic toxicity studies for micronucleus analysis, we orally administered phenacetin or B-mercaptopurine for 14 days to groups of six rats and compared their micronucleus frequencies to the bone marrow micronucleus frequencies of rats similarly treated for only 2 days. In the 14-day test, phenacetin significantly increased the frequency of micronucleated reticulocytes in peripheral blood at 500 mg/kg starting from day 9, and at 750 and 1500 mg/kg starting from day 6; 6-mercaptopurine gave a positive response at 20 mg/kg starting from day 6. Positive responses in the bone marrow assay were obtained at the same dose levels. In the 2-day test, micronucleated polychromatic erythrocyte frequencies increased significantly at 1000 and 2000 mg/kg for phenacetin, and at 50, 100, and 200 mg/kg for 6-mercaptopurine. These results suggest that micronucleus assays using peripheral blood from rats in subchronic animal studies of phenacetin and 6-mercaptopurine are feasible and at least as sensitive for the assessment of micronuclei as an acute bone marrow micronucleus test.
机译：为了检验使用亚慢性毒性研究中所用动物的大鼠外周血进行微核分析的适用性，我们对六只大鼠进行了口服非那西丁或B-巯基嘌呤治疗14天，并将它们的微核频率与大鼠的骨髓微核频率进行了比较治疗仅2天。在14天的测试中，非那西丁从第9天开始以500 mg / kg显着增加外周血中微核网织细胞的频率，从第6天开始以750和1500 mg / kg显着增加外周血中微核网织细胞的频率。从第6天开始，6-巯基嘌呤以20mg / kg给出阳性反应。在骨髓试验中以相同剂量水平获得阳性反应。在2天的测试中，非那西丁的微核多色红细胞频率在1000和2000 mg / kg时显着增加，而6-巯基嘌呤在50、100和200 mg / kg时显着增加。这些结果表明，在非扎西汀和6-巯基嘌呤的亚慢性动物研究中，使用大鼠外周血进行微核测定是可行的，并且至少与急性骨髓微核试验一样敏感。

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