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首页> 外文期刊>Mycologia >Local population structure of the wood decay ascomycete Daldinia loculata
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Local population structure of the wood decay ascomycete Daldinia loculata

机译：木材腐烂子囊菌Daldinia loculata的局部种群结构

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This paper presents a study of the local population structure of Daldinia loculata, a xylariaceous species frequently producing conidia and sexual stromata on fire-damaged deciduous host trees. We were able to show endophytical establishment of D. loculata by isolating mycelia from sound-looking wood of 8 out of 14 freshly felled, nan-burned trees. Mycelia were isolated from all 17 burnt trees in a burned forest site of central Sweden hosting stromata of D. loculata, and the distribution of genets in the stand was determined by using both vegetative incompatibility tests and restriction enzyme analysis of PCR amplified nuclear gene fragments as codominant molecular markers. In 14 of the trees we found only one genet of D. loculata per stem, and in three of the trees we found two genets per stem. Each genet was found only once in the stand, highlighting the relative importance of ascospores instead of conidia for dispersal to new substrata. Only one allele per locus was found in the mycelium isolated from the wood, suggesting that D. loculata grows vegetatively as haploid mycelia. Vegetative incompatibility and segregating molecular markers were observed in the progeny of single stromata, evidently a consequence of D. loculata being heterothallic. More than two alleles of a highly variable multiallelic locus were detected in each of two stromata when analyzing single ascospores from 14 to 16 perithecia, evenly distributed on the stromatal surface, suggesting that more than one mating event can be involved in stroma formation in D. loculata. Moreover most genotyped stromata of the same tree showed a distinct heterozygous genotype, indicating multiple matings of the relatively large, haploid, vegetative mycelium in the wood by several second, very small, individual mycelia.
机译：本文提出了对Daldinia loculata本地种群结构的研究，Daldinia loculata是经常在火灾中受损的落叶宿主树上产生分生孢子和有性层的木糖科物种。通过从14棵刚砍伐的楠木烧成的树木中，有8棵看上去很美的木材中分离出菌丝体，我们能够显示出D. loculata的内生菌的形成。从瑞典中部的一个烧毁森林的所有17棵烧毁的树木中分离出菌丝体，该树木位于D. loculata的地层中，通过营养不相容性测试和PCR扩增的核基因片段的限制性酶切分析，确定了林分的分布。共同的分子标记。在其中的14棵树中，每个茎只发现了一个D. loculata属，而在其中的三棵树中，每个茎中发现了两个基因。每个谱系仅在林分中被发现一次，突出了子囊孢子而不是分生孢子对于扩散到新基质的相对重要性。在从木材中分离出的菌丝体中，每个基因座仅发现一个等位基因，这表明D. loculata像单倍体菌丝体一样营养生长。在单个基质的子代中观察到营养不相容和分离的分子标记，这显然是D. loculata是异源的。分析14至16个周皮的单个子囊孢子时，在两个基质中的每个基质中均检测到两个以上高度可变的多等位基因，这些子囊均匀分布在气孔表面上，这表明在D的基质形成中可能涉及多个交配事件。 loculata。此外，同一棵树的大多数基因型基质均表现出独特的杂合基因型，表明木材中相对较大的单倍体营养菌丝体通过数秒（非常小）的单个菌丝体多次交配。

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