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The influence of nutrients on development, resting hyphae and aleuriospore induction of Thielaviopsis basicola.

机译:营养物质对基本型Thielaviopsis basicola的发育,静止菌丝和紫胶孢子诱导的影响。

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Two isolates of T. basicola, one from tobacco and one from cotton, were grown in crude host root extracts at varied dilutions. Aspects of hyphal morphology including hyphal diameter, hyphal length and degree of branching were positively correlated with the level of available nutrients under these conditions. Atypical hyphal forms were produced by T. basicola under conditions of nutrient stress, and these forms were similar to 2 cultural mutants previously reported. Secondary chlamydospores were also observed under nutrient stress conditions. Hyphal segments that possessed cytological and morphological features analogous to endoconidia and aleuriospores of T. basicola were observed during later stages of culture development under nutrient conditions favourable for growth and reproduction. These structures were termed resting hyphae, as T. basicola grew from them upon restoration of nutrients when other regions of hyphae were no longer viable. Natural and simulated depletion of nutrients in the culture environment following a period of vegetative growth induced aleuriospore production. Development of aleuriospores under these culture conditions showed that T. basicola can produce a substantial number of reproductive structures from nutrient reserves held within the existing thallus.
机译:在不同的稀释度下,在粗宿主根提取物中培养了两种分离的基础毛霉菌,一种来自烟草,一种来自棉花。在这些条件下,菌丝形态的各个方面(包括菌丝直径,菌丝长度和分支程度)与可利用的营养素水平呈正相关。非典型菌丝形式是由T. basicola在营养胁迫条件下产生的,这些形式类似于先前报道的2个文化突变体。在营养胁迫条件下也观察到次级衣原体孢子。在有利于生长和繁殖的营养条件下的培养发展的后期阶段,观察到了具有类似于内生分生孢子菌内生孢子和内生孢子菌的细胞学和形态学特征的菌丝节段。这些结构被称为静息菌丝,因为当其他菌丝区不再可行时,营养物质恢复后,巴氏锥虫便从中生长。经过一段时间的营养生长后,培养环境中营养素的自然消耗和模拟消耗会导致白粉虱的产生。在这些培养条件下,无孢子孢子的发育表明,T。basicola可以从现有菌体中保存的营养储备中产生大量的生殖结构。

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