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Atomic force microscopy study of the structure-function relationships of the biofilm-forming bacterium Streptococcus mutans

机译:生物膜形成细菌变形链球菌的结构-功能关系的原子力显微镜研究

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Atomic force microscopy (AFM) has garnered much interest in recent years for its ability to probe the structure, function and cellular nanomechanics inherent to specific biological cells. In particular, we have used AFM to probe the important structure-function relationships of the bacterium Streptococcus mutans. S. mutans is the primary aetiological agent In human dental caries (tooth decay), and is of medical importance due to the virulence properties of these cells in biofilm initiation and formation, leading to increased tolerance to antibiotics. We have used AFM to characterize the unique surface structures of distinct mutants of S. mutans. These mutations are located in specific genes that encode surface proteins, thus using AFM we have resolved characteristic surface features for mutant strains compared to the wild type. Ultimately, our characterization of surface morphology has shown distinct differences in the local properties displayed by various S. mutans strains on the nanoscale, which is imperative for understanding the collective properties of these cells in biofilrn formation.
机译:原子力显微镜(AFM)近年来因其探测特定生物细胞固有的结构,功能和细胞纳米力学的能力而备受关注。特别是,我们已经使用AFM来探测变形链球菌的重要结构-功能关系。变形链球菌是人类龋齿(蛀牙)的主要病因,并且由于这些细胞在生物膜形成和形成过程中的毒性特性而具有医学重要性,从而导致对抗生素的耐受性增加。我们已经使用原子力显微镜来表征变形链球菌不同突变体的独特表面结构。这些突变位于编码表面蛋白的特定基因中,因此与野生型相比,使用AFM,我们已解决了突变菌株的特征表面特征。最终,我们对表面形态的表征已显示出各种变形链球菌菌株在纳米尺度上表现出的局部特性上的明显差异,这对于了解这些细胞在生物膜形成中的集体特性至关重要。

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