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首页> 外文期刊>Natural product communications >Radical scavenging activity and total phenolic content of extracts of the root bark of Osyris lanceolata
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Radical scavenging activity and total phenolic content of extracts of the root bark of Osyris lanceolata

机译：柳树根皮提取物的自由基清除活性和总酚含量

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摘要

The root bark of Osyris lanceolata was screened for its antioxidant potential using three variations of the DPPH radical scavenging method viz a TLC-autographic assay, a semi-quantitative TLC assay, and a spectrophotometric assay. The total phenolic content was evaluated as gallic acid equivalents (GAE) using the Folin-Ciocalteu method. The powdered root bark was extracted sequentially with n-hexane, chloroform, methanol and 90% methanol/water. A separate supercritical fluid extraction (SFE) of the root bark was also carried out. The radical scavenging ability, as measured by IC_(50) values, was found to be 48.4 ± 0.4 and 49.5 ± 1.9 μ μg/mL for the 90% methanol/water and methanol extracts respectively, while those of the chloroform, n-hexane, and SFE extracts were found to be > 250 μg/mL. Under the same conditions, the values for the standards, ascorbic acid and gallic acid, were found to be 38.70 ± 0.08 and 2.86 ± 0.01 μg/mL respectively. The radical scavenging power of the five extracts correlated positively with the total phenolic content and was ranked in the following decreasing order: 90% methanol/water > methanol > chloroform > SFE > n-hexane with total phenolic contents of 290.2 ± 3.3, 271.0 ± 3.7, 74.4 ± 0.8, 62.5 ± 0.4 and 49.5 ± 1.9 mg GAE/g of dry extract, respectively. The 90% methanol/water and methanol extracts showed several components with high antioxidant activity displaying fast kinetics in both TLC assays, while the chloroform, SFE, and n-hexane extracts exhibited slow kinetics antioxidant activity.
机译：使用DPPH自由基清除方法的三种变体，即TLC-自动成像测定，半定量TLC测定和分光光度测定，筛选了Osyris lanceolata根皮的抗氧化能力。使用Folin-Ciocalteu方法将总酚含量评估为没食子酸当量（GAE）。依次用正己烷，氯仿，甲醇和90％甲醇/水萃取粉末状的根皮。还对根皮进行了单独的超临界流体萃取（SFE）。通过IC_（50）值测得的自由基清除能力，对于90％的甲醇/水和甲醇提取物，分别为48.4±0.4和49.5±1.9μg/ mL，而对于氯仿，正己烷则为，发现SFE提取物> 250μg/ mL。在相同条件下，抗坏血酸和没食子酸的标准值分别为38.70±0.08和2.86±0.01μg/ mL。五种提取物的自由基清除能力与总酚含量呈正相关，并按以下降序排列：90％甲醇/水>甲醇>氯仿> SFE>正己烷，总酚含量为290.2±3.3，271.0± GAE / g干提取物分别为3.7、74.4±0.8、62.5±0.4和49.5±1.9 mg GAE。 90％的甲醇/水和甲醇萃取物在两种TLC分析中均显示出具有高抗氧化活性的几种成分，显示出快速的动力学，而氯仿，SFE和正己烷萃取物则显示出较慢的动力学抗氧化活性。

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