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首页> 外文期刊>Biochimica et biophysica acta. Gene structure and expression >Involvement of the elicitor-induced gene OsWRKY53 in the expression of defense-related genes in rice
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Involvement of the elicitor-induced gene OsWRKY53 in the expression of defense-related genes in rice

机译：激发子诱导基因OsWRKY53参与水稻防御相关基因的表达

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摘要

We present a detailed characterization of the chitin oligosaccharide elicitor-induced gene OsWRKY53. OsWRKY53 was also induced in suspension-cultured rice cells by a fungal cerebroside elicitor and in rice plants by infection with the blast fungus Magnaporthe grisea. A fusion of OsWRKY53 with green fluorescent protein was detected exclusively in the nuclei of onion epidermal cells, and OsWRKY53 protein specifically bound to W-box elements. A transient assay using the particle bombardment method showed that OsWRKY53 is a transcriptional activator. A microarray analysis revealed that several defense-related genes, including pathogenesis-related protein genes such as PBZ1, were upregulated in rice cells overexpressing OsWRKY53. Finally, overexpression of OsWRKY53 in rice plants resulted in enhanced resistance to M. grisea. These results strongly suggest that OsWRKY53 is a transcription factor that plays important roles in elicitor-induced defense signaling pathways in rice.
机译：我们提出了几丁质寡糖激发子诱导基因OsWRKY53的详细表征。 OsWRKY53还通过真菌脑苷脂诱导剂在悬浮培养的水稻细胞中诱导，并通过感染稻瘟病菌Magnaporthe grisea诱导在水稻植物中诱导。仅在洋葱表皮细胞的细胞核中检测到OsWRKY53与绿色荧光蛋白的融合，并且OsWRKY53蛋白与W-box元件特异性结合。使用粒子轰击方法的瞬时分析表明，OsWRKY53是转录激活因子。基因芯片分析显示，在过量表达OsWRKY53的水稻细胞中，一些与防御相关的基因，包括与发病相关的蛋白质基因，例如PBZ1，被上调。最后，水稻植物中OsWRKY53的过表达导致对稻瘟病菌的抗性增强。这些结果强烈表明，OsWRKY53是一个转录因子，在水稻的诱导子诱导的防御信号通路中起着重要的作用。

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