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首页> 外文期刊>Nucleic Acids Research >NOVEL PATTERN OF DNA METHYLATION IN NEUROSPORA CRASSA TRANSGENIC FOR THE FOREIGN GENE HPH
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NOVEL PATTERN OF DNA METHYLATION IN NEUROSPORA CRASSA TRANSGENIC FOR THE FOREIGN GENE HPH

机译：外来基因HPH转基因神经CR的DNA甲基化新模式

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摘要

It has previously been reported that multiple copies of the hph gene integrated into the genome of Neurospora crassa are methylated at Hpall sites (CCGG) during the vegetative life cycle of the fungus, while hph genes integrated as single copies are not methylated. Furthermore, methylation is correlated with silencing of the gene. We report here the methylation state of cytosine residues of the major part of the promoter region of the hph gene integrated into the genome of the multiple copy strain HTA5.7 during the vegetative stage of the life cycle. Cytosine methylation is sequence dependent, but the sequence specificity is complex and is different from the sequence specificity known for mammals and plants (CpG and CpNpG). The pattern of DNA methylation reported here is very different from that measured after meiosis in Neurospora or in Ascobulus. After the sexual cycle in those two fungi all the cytosines of multiple stretches of DNA are heavily methylated. This indicates that the still unknown methyltransferase in Neurospora has a different specificity in the sexual and the vegetative stages of the life cycle or that there are different methyltransferases. The pattern of methylation reported here is also different from the pattern of cytosine methylation of transgenes of Petunia, the only pattern published until now in plants that has DNA methylation at cytosines which are not in the canonical sequences CpG and CpNpG.
机译：先前已有报道，在真菌的营养生命周期中，整合到克氏梭菌基因组中的hph基因的多个拷贝在Hpall位点（CCGG）被甲基化，而整合为单拷贝的hph基因未被甲基化。此外，甲基化与基因沉默相关。我们在这里报告在生命周期的营养阶段，hph基因启动子区域主要部分的胞嘧啶残基的甲基化状态，该基因整合到多拷贝菌株HTA5.7的基因组中。胞嘧啶甲基化是序列依赖性的，但是序列特异性是复杂的并且不同于哺乳动物和植物（CpG和CpNpG）已知的序列特异性。此处报道的DNA甲基化模式与减数分裂后在Neurospora或Ascobulus中测得的模式非常不同。在这两种真菌的有性循环之后，多个DNA片段的所有胞嘧啶都被高度甲基化。这表明Neurospora中仍未知的甲基转移酶在生命周期的性和营养阶段具有不同的特异性，或者存在不同的甲基转移酶。此处报道的甲基化模式也不同于矮牵牛转基因的胞嘧啶甲基化模式，这是迄今为止在植物中在胞嘧啶中具有DNA甲基化的植物中唯一的模式，而规范化序列CpG和CpNpG中没有。

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