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首页> 外文期刊>Cell motility and the cytoskeleton >Microtubule configurations and post-translational alpha-tubulin modifications during mammalian spermatogenesis.
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Microtubule configurations and post-translational alpha-tubulin modifications during mammalian spermatogenesis.

机译：哺乳动物精子发生过程中的微管构型和翻译后的α-微管蛋白修饰。

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摘要

The mechanisms underlying cell cycle progression and differentiation are tightly entwined with changes associated in the structure and composition of the cytoskeleton. Mammalian spermatogenesis is a highly intricate process that involves differentiation and polarization of the round spermatid. We found that pachytene spermatocytes and round spermatids have most of the microtubules randomly distributed in a cortical network without any apparent centrosome. The Golgi apparatus faces the acrosomal vesicle and some microtubules contact its surface. In round spermatids, at step 7, there is an increase in short microtubules around and over the nucleus. These microtubules are located between the rims of the acrosome and may be the very first sign in the formation of the manchette. This new microtubular configuration is correlated with the beginning of the migration of the Golgi apparatus from the acrosomal region towards the opposite pole of the cell. Next, the cortical microtubules form a bundle running around the nucleus perpendicular to the main axis of the cell. At later stages, the nuclear microtubules increase in size and a fully formed manchette appears at stage 9. On the other hand, acetylated tubulin is present in a few microtubules in pachytene spermatocytes and in the axial filament (precursor of the sperm tail) in round spermatids. Our results suggest that at step 7, the spermatid undergoes a major microtubular reordering that induces or allows organelle movement and prepares the cell for the formation of the manchette and further nuclear shaping. This new microtubular configuration is associated with an increase in short microtubules over the nucleus that may correspond to the initial step of the manchette formation. The new structure of the cytoskeleton may be associated with major migratory events occurring at this step of differentiation. Copyright 2000 Wiley-Liss, Inc.
机译：细胞周期进程和分化的基础机制与细胞骨架的结构和组成相关的变化紧密地交织在一起。哺乳动物的精子发生是一个高度复杂的过程，涉及圆形精子的分化和极化。我们发现，粗线精原细胞和圆形精子细胞的大多数微管随机分布在皮层网络中，而没有任何明显的中心体。高尔基体面对顶体囊泡，一些微管接触其表面。在圆形精子细胞中，在第7步，细胞核周围和上方的短微管增加。这些微管位于顶体的边缘之间，并且可能是在形成manchette的第一个迹象。这种新的微管构型与高尔基体从顶体区域向细胞的相反极迁移的开始有关。接下来，皮层微管形成一束围绕核垂直于细胞主轴延伸的束。在后期，核微管的大小增加，在第9阶段出现完全形成的手管。另一方面，乙酰化的微管蛋白存在于粗线精子细胞和轴向细丝（精子尾的前体）的一些微管中。精子。我们的结果表明，在第7步中，精子经历了主要的微管重排，从而诱导或允许细胞器运动，并为细胞的形成和进一步的核成形做好了准备。这种新的微管构型与核上短微管的增加有关，这可能对应于man管形成的初始步骤。细胞骨架的新结构可能与分化的这一阶段发生的主要迁徙事件有关。版权所有2000 Wiley-Liss，Inc.

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