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首页> 外文期刊>Cell motility and the cytoskeleton >Na+/Ca2+ exchanger modulates the flagellar wave pattern for the regulation of motility activation and chemotaxis in the ascidian spermatozoa.
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Na+/Ca2+ exchanger modulates the flagellar wave pattern for the regulation of motility activation and chemotaxis in the ascidian spermatozoa.

机译：Na + / Ca2 +交换剂可调节鞭毛波型，以调节海鞘精子的活力激活和趋化性。

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摘要

Ion channels and ion exchangers are known to be important participants in various aspects of sperm physiology, e.g. motility activation, chemotaxis, the maintenance of motility and the acrosome reaction in the sperm. We report here on a role of the K+ -independent Na+/Ca2+ exchanger (NCX) on ascidian sperm. Reverse-transcriptase PCR reveals that the NCX is expressed in the testis while immunoblotting and immunolocalization demonstrate that the NCX exists on the sperm in the ascidian Ciona savignyi and C. intestinalis. A potent blocker of the NCX, KB-R7943 was found to block sperm-activating and -attracting factor (SAAF)-induced motility activation, sperm motility and sperm chemotaxis. We further analyzed the effects of this blocker on motility parameters such as the flagellar waveform, curvature, beat frequency, amplitude and wavelength of the sperm flagella. Inhibition of the NCX caused two distinct effects: a low concentration of KB-R7943 induced symmetric bending, whereas a high concentration of KB-R7943 resulted in asymmetric flagellar bending. These findings suggest that the NCX plays important roles in the regulation of SAAF-induced sperm chemotaxis, motility activation and motility maintenance in the ascidian. This study provides new information toward an understanding of Ca2+ transport systems in sperm motility and chemotaxis.
机译：已知离子通道和离子交换剂是精子生理各个方面的重要参与者，例如活力激活，趋化，维持活力和精子顶体反应。我们在此报告了独立于K +的Na + / Ca2 +交换子（NCX）在海鞘精子上的作用。逆转录酶PCR显示NCX在睾丸中表达，而免疫印迹和免疫定位表明NCX存在于海鞘Ciona savignyi和C. intestinalis的精子中。发现NCX的强力阻滞剂KB-R7943可以阻断精子激活和吸引因子（SAAF）诱导的运动活化，精子运动和精子趋化性。我们进一步分析了该阻滞剂对运动参数的影响，例如鞭毛波形，曲率，搏动频率，精子鞭毛的振幅和波长。抑制NCX引起两个明显的影响：低浓度的KB-R7943引起对称弯曲，而高浓度的KB-R7943导致不对称鞭毛弯曲。这些发现表明，NCX在调节SAAF诱导的精子趋化性，运动性激活和维持性运动中起着重要作用。这项研究为了解Ca2 +转运系统对精子运动和趋化性提供了新的信息。

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