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Antioxidant properties and PC12 cell protective effects of APS-1, a polysaccharide from Aloe vera var. chinensis

机译:APS-1(一种来自芦荟的多糖)的抗氧化特性和PC12细胞保护作用。中华

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Through a combination of anion-exchange and repeated gel chromatographies, APS-1 was isolated from fresh leaves of Aloe vera L. var. chinensis (Haw.) Berger (an edible and medicinal plant widely cultivated and consumed in China) as a principal polysaccharide composed of mannose and glucose (ca. 18:5) with its molecular weight around 2.1 X 10(5). In a dose-dependent manner, APS-1 was demonstrated to be free radical scavenging in superoxide and hydroxyl radical assays, inhibitory to the copper-mediated oxidation of human low density lipoprotein (LDL), and protective against hydrogen peroxide (H2O2)-induced lesion to rat PC 12 cell (pheochromocytoma cell line). The result suggested that APS-1 could be of considerable preventive and therapeutic significance to some free radical associated health problems such as coronary heart ailments, Parkinson's and Alzheimer's diseases. Furthermore, the finding shed as well fresh light helpful for a better understanding of the health-benefiting potential of the edible plant consumed by the Chinese people for a couple of centuries. (c) 2005 Published by Elsevier Inc.
机译:通过阴离子交换和重复凝胶色谱的组合,从芦荟新鲜叶片中分离出APS-1。 (Haw。)Berger(一种在中国广泛种植和消费的食用和药用植物),是一种主要的多糖,由甘露糖和葡萄糖(约18:5)组成,分子量约为2.1 X 10(5)。以剂量依赖的方式,APS-1在超氧化物和羟基自由基测定中被证明具有清除自由基的作用,可抑制铜介导的人类低密度脂蛋白(LDL)的氧化,并能防止过氧化氢(H2O2)诱导损害大鼠PC 12细胞(嗜铬细胞瘤细胞系)。结果表明,APS-1对某些自由基相关的健康问题(如冠心病,帕金森氏症和阿尔茨海默氏病)具有重要的预防和治疗意义。此外,该发现还提供了新鲜的光线,有助于更好地了解中国人民食用了几个世纪的食用植物的健康潜力。 (c)2005年由Elsevier Inc.发布。

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