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首页> 外文期刊>Life sciences >Expression of tissue inhibitor of metalloproteinase-4 (TIMP-4) in endometrium and placenta of rhesus monkey (Macaca mulatta) during early pregnancy
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Expression of tissue inhibitor of metalloproteinase-4 (TIMP-4) in endometrium and placenta of rhesus monkey (Macaca mulatta) during early pregnancy

机译：早期妊娠恒河猴子宫内膜和胎盘中金属蛋白酶4（TIMP-4）组织抑制剂的表达

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摘要

The tissue inhibitors of metalloprotemases (TIMPs) are secreted as important regulators of the matrix metalloprotemases (MMPs). TIMP-4 is the most recently characterized member of the TIMTs family. In the present study, we examined the expression and localization of the TIMP-4 transcript and protein in endometrium and placenta of rhesus monkey (Macaca mulatta) on days 12, 18 and 26 of pregnancy using RT-PCR, in situ hybridization, and immunohistochemistry. The fragment of TIMP-4 gene from rhesus monkey uterine samples shared 95% identity with the corresponding region of human homologue. On day 12 of pregnancy, TIMP-4 mRNA was mainly expressed in the glandular and luminal epithelium. On days 18 and 26 of pregnancy, the expression of TIMP-4 mRNA tended to decline in glandular epithelium and there were strong staining in the placental villi. Furthermore, TIMP-4 mRNA was very faint or undetectable in the stromal cells, endothelial cells of arterioles and myometrium at any stages of pregnancy. The results of immunohistochemical analysis were similar to that of its mRNA. These findings indicate that TIMP-4 might play an important role in glandular secretion, endometrial tissue remodeling and invasion of the trophoblast cells by regulating MMPs in a localized manner in the uteri of rhesus monkey during early pregnancy. (c) 2005 Elsevier Inc. All rights reserved.
机译：金属蛋白酶的组织抑制剂（TIMPs）是基质金属蛋白酶（MMPs）的重要调节剂。 TIMP-4是TIMTs系列的最新特征。在本研究中，我们使用RT-PCR，原位杂交和免疫组化方法检测了妊娠12、18和26天恒河猴（猕猴）子宫内膜和胎盘中TIMP-4转录本和蛋白的表达和定位。来自恒河猴子宫样品的TIMP-4基因的片段与人类同源物的相应区域具有95％的同一性。在怀孕的第12天，TIMP-4 mRNA主要在腺和腔上皮中表达。在怀孕的第18和26天，TIMP-4 mRNA的表达在腺上皮中趋于下降，胎盘绒毛中有很强的染色。此外，TIMP-4 mRNA在妊娠的任何阶段在基质细胞，小动脉的内皮细胞和子宫肌层中都非常微弱或无法检测到。免疫组化分析的结果与其mRNA相似。这些发现表明，TIMP-4可能通过在妊娠早期的子宫中局部调节MMPs而在腺体分泌，子宫内膜组织重塑和滋养细胞侵袭中发挥重要作用。 （c）2005 Elsevier Inc.保留所有权利。

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