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Growth-active peptides are produced from alpha-lactalbumin and lysozyme

机译：由α-乳白蛋白和溶菌酶产生具有生长活性的肽

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We determined the growth-active domains of milk-growth factor (MGF), human (x-lactalbumin (HMLA) and human lysozyme (HMLZ), and their sequences. Fetal calf serum (FCS) showed inhibitors against proteases. The growth-stimulation of IMR90 cells in CG medium (free-serum) without FCS was induced in a dose-dependent manner up to 400 ng/mI of HMLA, HMLZ or chicken lysozyme (ChLZ), and also in a time-dependent manner until 48 It but was induced gradually until 1000 ng/mI of bovine (x-lactalbumin (BVLA). The HMLAL6-peptide (HMLAL6), a cleaved product from HMLA by Endpeptidase Lys C, was growth-stimulative. The sequence of HMLAL6 was matched to 35 amino-acid residues (from No. 59 to No. 93 of HMLA), owing to the sequences of HMLAL6R3, HMLAL6R5 and HMLAL6R7 after the reduction of HMLAL6. The sequences of the reduced peptides from MGFL7-peptide (MGFL7: a cleaved product from MGF by Endpeptidase lysine C matched to those of the peptides from HMLAL6, and were similarly identified as the partial sequence of HMLA (59-93, H2N-L.W.C.?.K./S.S.Q.V.P.Q.S.R.N.I.?.D.I.S.?. D.K./F.L. D.D.D.I.T.D.D.I.M.?.A.-COOH). The sequence of HMLZ is similar to that of HMLA. HMLZT7-peptide (HMLZT7), a cleaved product of HMLZ by trypsin, was confirmed to have growth-stimulating activity and it's sequence was partially identified as Y. W.?.N.D.G.K.T.P.G.A.V. N.A.?.H.L. owing to the results of HMLZT7R1 (reduction of HMLZT7) and HMLZA7R2 (reduction of HMLZA7-peptide (HMLZA7) cleaved product of HMLZ by Endpeptidase Arg C) and is accordingly the sequence from No. 63 to No. 97 of HMLZ. Therefore, the peptides produced from LA and LZ by proteolysis may play a role of growth-stimulation. (c) 2007 Elsevier Inc. All rights reserved.
机译：我们确定了牛奶生长因子（MGF），人（x-乳白蛋白（HMLA）和人溶菌酶（HMLZ）的生长活性域及其序列，胎牛血清（FCS）显示了针对蛋白酶的抑制剂。在不加FCS的CG培养基（游离血清）中IMR90细胞以剂量依赖性方式诱导高达400 ng / mI的HMLA，HMLZ或鸡溶菌酶（ChLZ），并以时间依赖性方式诱导直至48 It / min逐渐诱导至1000 ng / ml牛（x-乳清蛋白（BVLA）），HMLAL6肽（HMLAL6）是内肽酶Lys C从HMLA裂解的产物，具有生长刺激性，HMLAL6的序列与35个氨基酸酸残基（从HMLA的59号到93号），归因于HMLAL6还原后的HMLAL6R3，HMLAL6R5和HMLAL6R7序列。MGFL7肽的还原肽序列（MGFL7：MGF的裂解产物）末端肽酶赖氨酸C的序列与HMLAL6的那些序列相匹配，并被类似地鉴定为部分序列HMLA（59-93，H2N-L.W.C。？。K./S.S.Q.V.P.Q.S.R.N.I。？。D.I.S。？。 D.K./F.L。 D.D.D.I.T.D.I.M.?.A.-COOH）。 HMLZ的序列与HMLA的序列相似。证实HMLZT7肽（HMLZT7）是由胰蛋白酶裂解的HMLZ产物，具有生长刺激活性，其序列被部分鉴定为Y. W.?.N.D.G.K.T.P.G.A.V.。 N.A.?.H.L。由于HMLZT7R1（还原HMLZT7）和HMLZA7R2（还原HMLZA7肽（HMLZA7）被内肽酶Arg C切割HMLZ的产物）的结果，因此是HMLZ的编号63至97的序列。因此，通过蛋白水解从LA和LZ产生的肽可能起到刺激生长的作用。 （c）2007 Elsevier Inc.保留所有权利。

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