• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Increased apoptosis in U937 cells over-expressing lipocortin 1 (annexin I).
【24h】

Increased apoptosis in U937 cells over-expressing lipocortin 1 (annexin I).

机译：过度表达脂皮质激素1（膜联蛋白I）的U937细胞凋亡增加。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The potential involvement of endogenous lipocortin 1 in the process of cellular apoptosis, particularly in cells of the myelo-monocytic lineage, has been investigated. U937 cells were transfected either with an antisense or a sense DNA for lipocortin 1 and the stable clones 36.4AS clone (20-40% lower lipocortin 1 levels) and 15S (30% higher lipocortin 1 levels) were obtained. Cell apoptosis was induced by incubation with tumor necrosis factor-alpha: optimal responses were observed within a 24 h incubation period at a 5 ng/ml concentration. Apoptosis was assessed both morphologically, by annexin V binding and cell cycle analysis with propidium iodide. Whilst no consistent difference was seen between wild type cells and clone 36.4AS, a higher incidence of apoptosis (ranging from +30% to + 60%) was observed in the 15S clone. Release of arachidonic acid from loaded cells was promoted by 24 h incubation with the cytokine, and a higher degree of release was measured in the 15S clone. These data indicate that endogenous intracellular lipocortin 1 is involved in the promotion of apoptosis in cells of the myelo-monocytic derivation.
机译：已经研究了内源性脂皮质激素1在细胞凋亡过程中，特别是在骨髓单核细胞系细胞中的潜在参与。用反义或有义DNA转染U937细胞以检测脂皮质激素1，并获得稳定的克隆36.4AS克隆（脂皮质素1水平降低20-40％）和15S（脂皮质素1水平升高30％）。通过与肿瘤坏死因子-α孵育诱导细胞凋亡：在24 ng孵育时间内以5 ng / ml的浓度观察到最佳反应。通过膜联蛋白V结合和碘化丙啶的细胞周期分析在形态学上评估凋亡。尽管在野生型细胞和克隆36.4AS之间未观察到一致的差异，但在15S克隆中观察到了更高的凋亡发生率（从+ 30％到+ 60％）。通过与细胞因子一起孵育24小时，可以促进花生四烯酸从负载细胞中释放，并在15S克隆中检测到更高的释放度。这些数据表明内源性细胞内脂皮质激素1参与了骨髓单核细胞来源的细胞凋亡的促进。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号