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首页> 外文期刊>Life sciences >In vivo and in vitro antioxidant properties of furosemide.
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In vivo and in vitro antioxidant properties of furosemide.

机译：速尿的体内和体外抗氧化性能。

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The aim of this study was to investigate in vivo and in vitro antioxidant properties of furosemide. In vitro, human red blood cells were submitted to oxidative stress (AAPH), in absence or in presence of different concentrations of furosemide. Potassium efflux was measured in order to quantify the oxidative stress after the action of AAPH on red blood cells. Allophycocyanin assay was also used to investigate antioxidant capacities of furosemide. For the in vivo experiment, male Wistar rats were used. A control group (n = 5) was treated by a daily intraperitoneal injection of saline solution (0.2 ml); 2 other groups (J0 and J+) were treated for 7 days by one daily intraperitoneal injection of furosemide (0.10 mg/kg/day). In the J+group, the injection of furosemide was done one hour before the experiment, while in the J0 group the last injection of furosemide was done on the 6th day and an injection of saline was performed one hour before the experiment. On the day of experiment, a laparotomy was performed under general anesthesia and blood was collected from abdominal aorta. Oxidative stress and antioxidant capacities were evaluated on Wistar rat red blood cells and plasma. In vitro results (oxidative challenge with AAPH) showed that oxidative stress was decreased in presence of furosemide. This was due to a potent free radical scavenging effect of furosemide. In vivo studies confirmed that furosemide had antioxidant properties. These data may be of great relevance in clinical practice, considering the use of large doses of furosemide in patients presenting pathology involving the production of free radicals.
机译：这项研究的目的是研究呋塞米的体内和体外抗氧化特性。在体外，在不存在或存在不同浓度的速尿的情况下，人类红细胞都遭受氧化应激（AAPH）。测量钾外流以便量化AAPH对红细胞作用后的氧化应激。别藻蓝蛋白测定法还用于研究呋塞米的抗氧化能力。对于体内实验，使用雄性Wistar大鼠。对照组（n = 5）通过每天腹腔注射生理盐水（0.2 ml）进行治疗;通过每天一次腹膜内注射速尿（0.10 mg / kg /天），将另外2组（J0和J +）治疗7天。在J +组中，速尿注射是在实验前一小时完成的，而在J0组中，最后尿液是在第六天进行速尿的注射，而盐水是在实验前一小时进行的。实验当天，在全身麻醉下进行剖腹手术，并从腹主动脉中收集血液。在Wistar大鼠红细胞和血浆上评估了氧化应激和抗氧化能力。体外研究结果（用AAPH进行氧化攻击）显示，在存在速尿的情况下氧化应激降低。这归因于速尿的有效自由基清除作用。体内研究证实速尿具有抗氧化性能。考虑到在表现出涉及自由基产生的病理的患者中使用大剂量的速尿，这些数据在临床实践中可能具有重要意义。

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