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首页> 外文期刊>Life sciences >IDENTIFICATION, CHARACTERIZATION AND LOCALIZATION OF CORTICOTROPIN-RELEASING HORMONE RECEPTORS IN HUMAN PLACENTA
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IDENTIFICATION, CHARACTERIZATION AND LOCALIZATION OF CORTICOTROPIN-RELEASING HORMONE RECEPTORS IN HUMAN PLACENTA

机译：人类胎盘中释放结肠皮质激素的激素受体的鉴定，表征和定位

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The purpose of this work was to identify, characterise, and localise specific CRH binding sites in whole vaginally delivered term human placental membranes as well as in dispersed and purified trophoblastic membranes. We have found that whole placenta membranes contained specific and saturable CRH-binding sites. Maximum specific binding was obtained at pH 7.2-7.4, 22 C, for 2 h. The order of potency of CRH analogs was h/rCRH>alpha-helCRH>oCRH. Scatchard analysis revealed a single population of high affinity binding sites with a dissociation constant of 1.25 nM and maximum binding capacity of 119 fmol/mg of protein. Purified syncytiotrophoblast membranes contained high affinity CRH binding sites exhibiting the same dissociation constant and maximum binding capacity as whole placental membranes, suggesting that the CRH binding sites are expressed almost exclusively by the syncytiotrophoblast. Our findings indicate that CRH binding sites in the human placenta are exhibit similar characteristics to that described for anterior pituitary, brain and adrenals. [References: 21]
机译：这项工作的目的是在整个阴道输送的足月人胎盘膜以及分散和纯化的滋养层膜中鉴定，表征和定位特定的CRH结合位点。我们发现整个胎盘膜包含特定的和饱和的CRH结合位点。在22℃的pH 7.2-7.4下获得最大的特异性结合2小时。 CRH类似物的效力顺序为h / rCRH>α-helCRH> oCRH。斯卡查德分析显示了高亲和力结合位点的单一群体，其解离常数为1.25 nM，最大结合能力为119 fmol / mg蛋白质。纯化的合体滋养层细胞膜含有高亲和力的CRH结合位点，展现出与整个胎盘膜相同的解离常数和最大结合能力，这表明CRH结合位点几乎只由合体滋养层细胞表达。我们的发现表明，人胎盘中的CRH结合位点表现出与垂体前叶，大脑和肾上腺所描述的特征相似的特征。 [参考：21]

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