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首页> 外文期刊>Life sciences >In vitro prolactin release from pituitaries of ovariectomized, estradiol-treated Holtzman rats: a direct comparison of dispersed cells and tissue explants.
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In vitro prolactin release from pituitaries of ovariectomized, estradiol-treated Holtzman rats: a direct comparison of dispersed cells and tissue explants.

机译：从卵巢切除，雌二醇治疗的Holtzman大鼠的垂体中释放体外催乳激素：分散细胞和组织外植体的直接比较。

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摘要

In this study we compared prolactin (PRL) release from pituitary explants with that from dispersed cells from two regions of anterior pituitaries obtained from ovariectomized rats treated for 7 days with estradiol. Pituitaries were cut horizontally into dorsal (or inner) and ventral (or outer) zones. One half of each these regions was enzymatically dispersed to cells; the other half was bisected and examined as tissue explants. The dispersed cells and explants were perifused in parallel over a 2 hour period with dopamine (1 microM) present for the first hour and absent during the second hour. Prolactin released into the perifusion medium was measured by radioimmunoassay. Tissue explants secreted more total prolactin than did cells in the presence or absence of dopamine and the pattern of release was sustained compared to the more transient release observed in dispersed cells. However, the response to dopamine withdrawal was not different between cells and explants when expressed as a percent change from respective controls. The results suggest that organization and architecture of the rat anterior pituitary may be important factors for overall prolactin production, but in terms of relative responsiveness to dopamine and its withdrawal, dispersed cells and tissue explants appear equivalent.
机译：在这项研究中，我们比较了垂体外植体的催乳素（PRL）释放与来自经雌二醇处理7天的去卵巢大鼠的垂体前叶两个区域的分散细胞释放。将垂体水平切成背侧（或内部）和腹侧（或外部）区域。这些区域中的每个区域的一半都通过酶分散到细胞中。另一半一分为二并检查为组织外植体。分散的细胞和外植体在2小时内与多巴胺（1 microM）并行灌注，第一个小时存在，第二个小时则没有。通过放射免疫测定法测定释放到灌注液中的催乳激素。与在多巴胺存在或不存在的情况下相比，组织外植体分泌的总催乳素比细胞分泌更多的催乳激素，并且与在分散细胞中观察到的更短暂的释放相比，释放的模式得以维持。然而，当以相对于各自对照的百分比变化表示时，细胞和外植体之间对多巴胺戒断的反应没有差异。结果表明，大鼠垂体前叶的组织和结构可能是产生催乳素总量的重要因素，但就对多巴胺及其戒断的相对反应而言，分散的细胞和组织外植体似乎是等效的。

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