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Evaluation of the pituitary-testicular function during experimental nephrosis.

机译：评估实验性肾病期间垂体-睾丸功能。

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To investigate the pituitary-testicular function in nephrotic rats, a sequence of experiments was undertaken in adult male rats after a single dose of puromycin aminonucleoside (PAN). Endocrine modifications were evaluated chronologically throughout the experimental disease in order to determine the appearance of hormone alterations which lead to the axis dysfunction. Serum concentration of LH, FSH, androstenedione, total and free testosterone, estradiol as well as urine testosterone were measured by specific RIAs on days 3, 7 and 10 after treatment on nephrotic and control groups. Prolactin was also evaluated on day 10. Likewise, total weight of various androgen responsive tissues from both groups was recorded, and the number of androgen receptor (AR) binding sites were determined. To know the functional status of the hipophyseal-testicular unit, groups of nephrotic and control rats were stimulated with LHRH (300 ng/100 g b.w.) or with one or four doses of hCG (8 UI), respectively. Additionally, the relative in vitro biological activity of FSH from nephrotic and control rats before and after LHRH stimulus was determined. The results from the hormonal profile revealed clear endocrine disorders characterized by a progressive diminution of all serum hormones except prolactin and urine testosterone, which remained unmodified. The weight of the main androgen responsive tissues, the ventral prostate and the seminal vesicle, decreased parallelly to androgen diminution. The binding analysis of AR shows a significant elevation of the available androgen sites in all analyzed tissues except kidney and hypothalamus. The secretion of LH and FSH from nephrotic animals after LHRH administration was lower than that from intact animals at the registered times. Interestingly, the biological activity of FSH from nephrotic rats was not detectable at both, before and after LHRH administration. Testicular response to hCG stimuli, in terms of testosterone synthesis was not significantly different in the two groups analyzed with respect to the intact animals. By contrast, no response was observed in terms of estradiol production at either one or four doses of hCG. On the whole, the results presented herein allow us to conclude that experimental nephrosis has a harmful effect on the pituitary-testicular axis, and strongly suggests that the endocrine dysfunction is initiated at the hypophyseal level; even though a specific testicular damage is also present.
机译：为了研究肾病大鼠的垂体-睾丸功能，单剂量嘌呤霉素氨基核苷（PAN）后在成年雄性大鼠中进行了一系列实验。在整个实验疾病中，按时间顺序对内分泌修饰进行评估，以确定是否出现导致轴功能障碍的激素改变。肾病组和对照组在治疗后第3、7和10天，通过特定的RIA测量LH，FSH，雄烯二酮，总和游离睾丸激素，雌二醇以及尿液睾丸激素的血清浓度。在第10天也评估了催乳素。同样，记录了两组中各种雄激素应答组织的总重量，并确定了雄激素受体（AR）结合位点的数量。为了了解马蹄s睾丸单位的功能状态，分别用LHRH（300 ng / 100 g b.w.）或一或四剂hCG（8 UI）刺激肾病和对照组大鼠。另外，测定了LHRH刺激前后来自肾病和对照大鼠的FSH的相对体外生物学活性。荷尔蒙特征的结果显示出明显的内分泌失调，其特征是除催乳素和尿液睾丸激素外，所有血清激素都逐渐减少，这些激素仍未改变。主要的雄激素反应组织，腹侧前列腺和精囊的重量与雄激素的减少平行地减少。 AR的结合分析显示，除肾脏和下丘脑外，所有分析组织中可用的雄激素位点均显着升高。 LHRH给药后，肾病动物的LH和FSH分泌在记录的时间内低于完整动物的分泌。有趣的是，在给予LHRH之前和之后，都无法检测到来自肾病大鼠的FSH的生物学活性。相对于完整动物，在分析的两组中，就睾丸激素合成而言，对hCG刺激的睾丸反应无显着差异。相比之下，就一剂或四剂hCG的雌二醇产生而言，未观察到反应。总体而言，本文提供的结果使我们可以得出结论，实验性肾病对垂体-睾丸轴具有有害作用，并强烈暗示内分泌功能障碍是在垂体水平开始的。即使也存在特定的睾丸损伤。

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