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首页> 外文期刊>Cellular immunology >Cyclophosphamide induces bone marrow to yield higher numbers of precursor dendritic cells in vitro capable of functional antigen presentation to T cells in vivo.
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Cyclophosphamide induces bone marrow to yield higher numbers of precursor dendritic cells in vitro capable of functional antigen presentation to T cells in vivo.

机译：环磷酰胺在体外诱导骨髓产生更多数量的前体树突状细胞，能够将抗原功能性地呈递给体内T细胞。

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摘要

We have shown recently that cyclophosphamide (CTX) treatment induced a marked increase in the numbers of immature dendritic cells (DCs) in blood, coinciding with enhanced antigen-specific responses of the adoptively transferred CD8(+) T cells. Because this DC expansion was preceded by DC proliferation in bone marrow (BM), we tested whether BM post CTX treatment can generate higher numbers of functional DCs. BM was harvested three days after treatment of C57BL/6 mice with PBS or CTX and cultured with GM-CSF/IL-4 in vitro. Compared with control, BM from CTX-treated mice showed faster generation and yielded higher numbers of DCs with superior activation in response to toll-like receptor (TLR) agonists. Vaccination with peptide-pulsed DCs generated from BM from CTX-treated mice induced comparable adjuvant effects to those induced by control DCs. Taken together, post CTX BM harbors higher numbers of DC precursors capable of differentiating into functional DCs, which be targeted to create host microenvironment riches in activated DCs upon treatment with TLR agonists.
机译：我们最近显示，环磷酰胺（CTX）处理引起血液中未成熟树突状细胞（DC）数量的显着增加，这与过继转移的CD8（+）T细胞的抗原特异性反应增强有关。因为此DC扩展之前是骨髓（BM）中DC增殖，所以我们测试了CTX治疗后BM是否可以产生更多数量的功能DC。用PBS或CTX处理C57BL / 6小鼠后三天收获BM，并在体外用GM-CSF / IL-4培养。与对照组相比，经CTX处理的​​小鼠的BM显示出更快的生成速度，并产生了更多的DC，具有对Toll样受体（TLR）激动剂的超强激活。用CTX处理的​​小鼠的BM产生的肽脉冲DC进行疫苗接种后，可产生与对照DC诱导的类似的佐剂作用。两者合计，后CTX BM包含更多数量的能够分化为功能性DC的DC前体，这些前体的目标是在通过TLR激动剂处理后在激活的DC中产生丰富的宿主微环境。

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