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In vivo recombination as a tool to generate molecular diversity in phage antibody libraries

机译:体内重组作为在噬菌体抗体库中产生分子多样性的工具

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摘要

The creation of diversity in populations of polypeptides has become an important tool in the derivation of polypeptides with useful characteristics. This requires efficient methods to create diversity coupled with methods to select polypeptides withdesired properties. In this review we describe the use of in vivo recombination as a powerful way to generate diversity. The novel principles for the recombination process and several applications of this process for the creation of phage antibody libraries are described. The advantage and disadvantages are discussed and possible future exploitation presented.
机译:多肽种群多样性的产生已成为衍生具有有用特性的多肽的重要工具。这需要产生多样性的有效方法,以及选择具有所需特性的多肽的方法。在这篇综述中,我们描述了体内重组作为产生多样性的有效途径。描述了重组过程的新颖原理以及该过程在建立噬菌体抗体文库中的几种应用。讨论了优缺点,并提出了可能的未来利用方法。

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