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Nitrogen-doped graphene quantum dots-based fluorescent probe for the sensitive turn-on detection of glutathione and its cellular imaging

机译：氮掺杂石墨烯量子点荧光探针用于谷胱甘肽的灵敏开启检测及其细胞成像

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In this paper, highly blue-luminescent nitrogen-doped graphene quantum dots (N-GQDs) are synthesized by a facile one-step hydrothermal treatment of citric acid and dicyandiamide. A quantum yield (QY) as high as 32.4% is achieved at an excitation wavelength of 350 nm. It is found that such N-GQDs with a high QY can be used as efficient fluorescent probes for the detection of glutathione (GSH). In the detection, the photoluminescence (PL) intensity of the N-GQDs could be quenched by mercuric ions due to the strong electrostatic interaction and electron transfer between N-GQDs and Hg(II). Upon the addition of GSH, the PL intensity of N-GQDs can be recovered owing to the preferred combination of Hg(II) and GSH by forming a Hg(II)-S bond. Under optimal conditions, this fluorescence turn-on sensing system exhibits excellent sensitivity and selectivity for GSH determination with a detection limit of 87 nM. Importantly, the N-GQDs-Hg(II) system can be successfully applied for visualizing the intracellular GSH in live HeLa cells due to bright luminescence, low cytotoxicity and good biocompatibility.
机译：在本文中，通过柠檬酸和双氰胺的一步一步水热处理，合成了高度蓝光的氮掺杂石墨烯量子点（N-GQDs）。在350 nm的激发波长下，量子产率（QY）高达32.4％。已经发现具有高QY的这种N-GQD可用作检测谷胱甘肽（GSH）的有效荧光探针。在检测中，由于N-GQD与Hg（II）之间的强静电相互作用和电子转移，N-GQD的光致发光（PL）强度可以被汞离子猝灭。加入GSH后，由于Hg（II）和GSH的优选组合，通过形成Hg（II）-S键，可以回收N-GQD的PL强度。在最佳条件下，此荧光灯开启传感系统对GSH的测定具有极好的灵敏度和选择性，检测限为87 nM。重要的是，N-GQDs-Hg（II）系统具有亮的发光，低的细胞毒性和良好的生物相容性，因此可以成功地用于可视化活HeLa细胞中的细胞内GSH。

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