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首页> 外文期刊>Biological chemistry >Heparinase selectively sheds heparan sulphate from the endothelial glycocalyx.
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Heparinase selectively sheds heparan sulphate from the endothelial glycocalyx.

机译:肝素酶选择性地从内皮糖萼中排出硫酸乙酰肝素。

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Abstract A healthy vascular endothelium is coated by the endothelial glycocalyx. Its main constituents are transmembrane syndecans and bound heparan sulphates. This structure maintains the physiological endothelial permeability barrier and prevents leukocyte and platelet adhesion, thereby mitigating inflammation and tissue oedema. Heparinase, a bacterial analogue to heparanase, is known to attack the glycocalyx. However, the exact extent and specificity of degradation is unresolved. We show by electron microscopy, immunohistological staining and quantitative measurements of the constituent parts, that heparinase selectively sheds heparan sulphate from the glycocalyx, but not the syndecans.
机译:摘要健康的血管内皮被内皮糖萼包覆。它的主要成分是跨膜合成多糖和结合的硫酸乙酰肝素。这种结构保持了生理性内皮渗透性屏障,并防止白细胞和血小板粘附,从而减轻了炎症和组织水肿。肝素酶是乙酰肝素酶的细菌类似物,已知会攻击糖萼。但是,降解的确切程度和特异性尚未解决。我们通过电子显微镜,免疫组织学染色和组成部分的定量测量表明,肝素酶选择性地从糖萼中脱落了硫酸乙酰肝素,但没有多糖。

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