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Microwave-assisted derivatization for fast and efficient analysis of saccharides on disposable microchips

机译：微波辅助衍生化可快速高效地分析一次性微芯片上的糖类

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摘要

For rapid and low-cost determination of saccharides with microchip electrophoresis, a domestic microwave oven was used to perform the derivatization of saccharides with 8-aminonaphthalene-1,3,6-trisulfonate (ANTS) for laser-induced fluorescence detection with a 405 nm laser diode. The influence of the reaction conditions was systematically examined. Derivatization of xylose, glucose and lactose was fulfilled within 3 min under the optimized condition. With appropriate selection of the separation medium, these model analytes could be resolved within 30 s in borate solutions containing hydroxypropyl cellulose using disposable cyclic olefin copolymer microchips. Theoretical plate numbers of 1.0 x 10(6) m(-1) and limits of detection of 0.15-0.23 mu M were achieved. Peak areas were linear up to 1000 mM for all analytes. The applicability of the proposed method was verified by the determination of glucose in human urine and serum. Separation of oligosaccharides from konjac powder was also demonstrated. The domestic microwave oven, the cheap laser diode and disposable microchips used here make the method an economical way for rapid analysis of saccharides.
机译：为了通过微芯片电泳快速，低成本地测定糖类，使用了家用微波炉用8-氨基萘-1,3,6-三磺酸盐（ANTS）进行糖的衍生化，用于405 nm激光诱导的荧光检测激光二极管。系统地检查了反应条件的影响。在优化的条件下，木糖，葡萄糖和乳糖的衍生化在3分钟内完成。通过适当选择分离介质，可以使用一次性环烯烃共聚物微芯片在30秒内将这些模型分析物解析为含有羟丙基纤维素的硼酸盐溶液。理论塔板数为1.0 x 10（6）m（-1），检出限为0.15-0.23μM。所有分析物的峰面积均为线性，最高可达1000 mM。通过测定人尿和血清中的葡萄糖，验证了该方法的适用性。还证实了从魔芋粉中分离寡糖。此处使用的家用微波炉，便宜的激光二极管和一次性微芯片使该方法成为快速分析糖类的经济方法。

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