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首页> 外文期刊>Biological chemistry >Somatotropic gene response to recombinant growth hormone treatment in buffalo leucocytes.
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Somatotropic gene response to recombinant growth hormone treatment in buffalo leucocytes.

机译：水牛白细胞对重组生长激素治疗的促生长基因反应。

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摘要

The use of recombinant bovine growth hormone (rbGH) to increase milk yield in cows is banned in some countries. In others, where it is authorised, it has triggered harsh debates on labelling of dairy products. If many studies have been performed on bovines, there is a lack of information on buffaloes, which are sometimes treated with rbGH and re-present an important economical resource for dairy products in some countries. Analytical methods with legal value for surveillance of rbGH treatments do not yet exist. Research on gene expression biomarkers is one of the most promising approaches to this purpose. For this reason, we treated five buffaloes for 10 weeks with a sustained-release formulation of rbGH and analysed the response of 20 somatotropic axis genes in leucocytes by real-time polymerase chain reaction. Overall changes in gene expression levels were of low magnitude and sometimes affected by the 'time' factor. Only the IGFBP-1 gene showed a significant under-expression (about two-fold; p <0.001) in treated animals. Taken together, these results give evidence that expression analysis of the somatotropic axis genes in leucocytes is little helpful for discrimination of rbGH-treated buffaloes, but do not exclude that another array of genes could provide useful patterns of variation.
机译：在某些国家，禁止使用重组牛生长激素（rbGH）提高奶牛的产奶量。在其他获得授权的地方，它引发了关于乳制品标签的激烈辩论。如果对牛进行了许多研究，则缺少有关水牛的信息，水牛有时用rbGH处理，在某些国家中再次代表着重要的奶制品经济来源。尚无用于监测rbGH治疗的具有法律价值的分析方法。基因表达生物标志物的研究是实现此目的的最有前途的方法之一。因此，我们用rbGH的缓释制剂对五个水牛进行了10周的治疗，并通过实时聚合酶链反应分析了白细胞中20个生长激素轴基因的响应。基因表达水平的总体变化幅度很小，有时受“时间”因素影响。在治疗的动物中，只有IGFBP-1基因显示出明显的表达不足（约两倍; p <0.001）。综上所述，这些结果提供了证据，即白细胞中的促生长轴基因的表达分析对分辨rbGH处理的水牛几乎没有帮助，但不排除其他基因阵列可以提供有用的变异模式。

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