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Optimization of supercritical fluid extraction of lycopene from tomato skin with central composite rotatable design model

机译：中心复合可旋转设计模型优选超临界流体萃取番茄皮中番茄红素。

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摘要

Response surface methodology using the central composite rotatetable design (CCRD) model was used to optimize parameters for supercritical carbon dioxide extraction of lycopene (C_(40)H_(56)) from dried tomato skin. The CCRD consisting of three-factored factorial design with two levels was used in this study. The factors used were temperature of the extraction chamber (40 and 70 °C), pressure of the extraction fluid (25 and 45 MPa), and modifier concentration (5 and 15%). Judged by the lack-of-fit-test, coefficient of determination and the standard errors results from the analysis of variance have shown the model to be adequate. The linear, quadratic and cross-effects were 0.58, 0.28 and 0.05, respectively. The independent variables have significantly (p < 0.05) influenced the extraction of all trans-lycopene from tomato skin. Although, no significant (p > 0.05) individual effect of modifier concentration shown, a synergetic effect was observed. A second-degree polynomial equation was developed from a response surface analysis for all trans-lycopene yield and the highest yield was predicted at 62 °C, 45 MPa (450 bar) and 14% temperature, pressure and modifier concentration, respectively and the recovery of all trans-lycopene was 33%.
机译：使用中央复合转台设计（CCRD）模型的响应面方法用于优化从干燥番茄皮中超临界二氧化碳萃取番茄红素（C_（40）H_（56））的参数。在这项研究中使用了由两级三因子析因设计组成的CCRD。使用的因素是萃取室的温度（40和70℃），萃取液的压力（25和45 MPa）以及改性剂浓度（5％和15％）。通过缺乏拟合检验判断，确定系数和方差分析得出的标准误差表明该模型是适当的。线性效应，二次效应和交叉效应分别为0.58、0.28和0.05。自变量显着（p <0.05）影响了番茄皮中所有反式番茄红素的提取。尽管没有显示出明显的（p> 0.05）修饰剂浓度单独作用，但观察到了协同作用。通过响应面分析得出了所有反式番茄红素收率的二次多项式方程，预测最高收率分别在62°C，45 MPa（450 bar）和14％的温度，压力和改性剂浓度下进行，回收率反式番茄红素的比例为33％。

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