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首页> 外文期刊>Biomaterials >Preferential cell attachment to nitrogen-doped diamond-like carbon (DLC:N) for the measurement of quantal exocytosis.
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Preferential cell attachment to nitrogen-doped diamond-like carbon (DLC:N) for the measurement of quantal exocytosis.

机译：优先细胞附着在氮掺杂的类金刚石碳（DLC：N）上，用于定量胞吐作用的测量。

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摘要

Electrochemical measurement of transmitter or hormone release from individual cells on microchips has applications both in basic science and drug screening. High-resolution measurement of quantal exocytosis requires the working electrode to be small (cell-sized) and located in immediate proximity to the cell. We examined the ability of candidate electrode materials to promote the attachment of two hormone-secreting cell types as a mechanism for targeting cells for to recording electrodes with high precision. We found that nitrogen-doped diamond-like carbon (DLC:N) promoted cell attachment relative to other materials tested in the rank order of DLC:N>In(2)O(3)/SnO(2) (ITO), Pt>Au. In addition, we found that treating candidate electrode materials with polylysine did not increase attachment of chromaffin cells to DLC:N, but promoted cell attachment to the other tested materials. We found that hormone-secreting cells did not attach readily to Teflon AF as a potential insulating material, and demonstrated that patterning of Teflon AF leads to selective cell targeting to DLC:N "docking sites". These results will guide the design of the next generation of biochips for automated and high-throughput measurement of quantal exocytosis.
机译：电化学测量微芯片上单个细胞中递质或激素释放的方法在基础科学和药物筛选中都有应用。定量胞吐作用的高分辨率测量要求工作电极小（细胞大小），并紧邻细胞。我们检查了候选电极材料促进两种分泌激素的细胞类型附着的能力，以此作为将细胞靶向至高精度记录电极的机制。我们发现相对于按DLC：N> In（2）O（3）/ SnO（2）（ITO），Pt的等级顺序测试的其他材料，氮掺杂的类金刚石碳（DLC：N）促进了细胞附着。 >金。此外，我们发现用聚赖氨酸处理候选电极材料不会增加嗜铬细胞对DLC：N的附着，但会促进细胞对其他测试材料的附着。我们发现，分泌激素的细胞并不容易附着在Teflon AF上作为潜在的绝缘材料，并证明了Teflon AF的图案形成导致选择性地将细胞靶向DLC：N“停靠位点”。这些结果将指导用于定量胞吐的自动化和高通量测量的下一代生物芯片的设计。

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