• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Parasitology >Expression of the histone chaperone SET/TAF-I beta during the strobilation process of Mesocestoides corti (Platyhelminthes, Cestoda)
【24h】

Expression of the histone chaperone SET/TAF-I beta during the strobilation process of Mesocestoides corti (Platyhelminthes, Cestoda)

机译：组蛋白伴侣SET / TAF-I beta在短鞭毛虫（Psotyhelminthes，Cestoda）的增粗过程中的表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The histone chaperone SET/TAF-I beta is implicated in processes of chromatin remodelling and gene expression regulation. It has been associated with the control of developmental processes, but little is known about its function in helminth parasites. In Mesocestoides corti, a partial cDNA sequence related to SET/TAF-I beta was isolated in a screening for genes differentially expressed in larvae (tetrathyridia) and adult worms. Here, the full-length coding sequence of the M. corti SET/TAF-I beta gene was analysed and the encoded protein (McSET/TAF) was compared with orthologous sequences, showing that McSET/TAF can be regarded as a SET/TAF-I beta family member, with a typical nucleosome-assembly protein (NAP) domain and an acidic tail. The expression patterns of the McSET/TAF gene and protein were investigated during the strobilation process by RT-qPCR, using a set of five reference genes, and by immunoblot and immunofluorescence, using monospecific polyclonal antibodies. A gradual increase in McSET/TAF transcripts and McSET/TAF protein was observed upon development induction by trypsin, demonstrating McSET/TAF differential expression during strobilation. These results provided the first evidence for the involvement of a protein from the NAP family of epigenetic effectors in the regulation of cestode development.
机译：组蛋白伴侣SET /TAF-1β参与染色质重塑和基因表达调节的过程。它与发育过程的控制有关，但对其在蠕虫寄生虫中的功能了解甚少。在Me皮中，在筛选幼虫（四甲虫）和成虫中差异表达的基因时，分离出了与SET / TAF-1β相关的部分cDNA序列。在这里，分析了M. corti SET / TAF-I beta基因的全长编码序列，并将编码的蛋白（McSET / TAF）与同源序列进行了比较，表明McSET / TAF可被视为SET / TAF。 -Iβ家族成员，具有典型的核小体组装蛋白（NAP）结构域和酸性尾巴。通过使用一组五个参考基因的RT-qPCR在分步过程中研究McSET / TAF基因和蛋白质的表达模式，并使用单特异性多克隆抗体通过免疫印迹和免疫荧光进行了研究。胰蛋白酶诱导发育后，观察到McSET / TAF转录本和McSET / TAF蛋白逐渐增加，这表明在粗壮过程中McSET / TAF差异表达。这些结果提供了来自NAP表观遗传效应子家族的蛋白质参与est的发育调控的第一个证据。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号