• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Parasitology >A heterologous prime-boost vaccination regime using DNA and a vaccinia virus, both expressing GRA4, induced protective immunity against Toxoplasma gondii infection in mice.
【24h】

A heterologous prime-boost vaccination regime using DNA and a vaccinia virus, both expressing GRA4, induced protective immunity against Toxoplasma gondii infection in mice.

机译：使用均表达GRA4的DNA和牛痘病毒的异源初免-加强疫苗接种方案可诱导小鼠抗弓形虫感染的保护性免疫。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

SUMMARYThe dense granule antigen 4 (GRA4) is known as an immundominant antigen of Toxoplasma gondii and, therefore, is considered as a vaccine candidate. For further evaluation of its vaccine effect, a recombinant plasmid and vaccinia virus, both expressing GRA4, were constructed, and a heterologous prime-boost vaccination regime was performed in a mouse model. The mice immunized with the heterologous prime-boost vaccination regime showed a high level of specific antibody response against GRA4 and a significantly high level of gamma interferon (IFN-gamma) production and survived completely against a subsequent challenge infection with a lethal dose of T. gondii. In addition, the formation of cysts was inhibited in the mice vaccinated with the heterologous prime-boost vaccination regime. These results demonstrate that the heterologous prime-boost vaccination regime using DNA and a vaccinia virus, both expressing GRA4, could induce both humoral and cellular immune responses and provide effective protection against lethal acute and chronic T. gondii infections in mice.
机译：发明内容致密颗粒抗原4（GRA4）被称为弓形虫的免疫优势抗原，因此被认为是候选疫苗。为了进一步评估其疫苗效果，构建了均表达GRA4的重组质粒和牛痘病毒，并在小鼠模型中进行了异源初免-加强免疫接种方案。用异源初免-加强免疫接种方案免疫的小鼠显示出高水平的针对GRA4的特异性抗体反应和显着高水平的γ干扰素（IFN-γ）产生，并且在随后的致死剂量的T感染中完全存活。贡迪另外，在用异源初免-加强疫苗接种方案接种的小鼠中，囊肿的形成被抑制。这些结果表明，使用同时表达GRA4的DNA和牛痘病毒的异源初免-加强疫苗接种方案可以诱导体液和细胞免疫应答，并提供针对小鼠致命的急性和慢性弓形虫感染的有效保护。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号