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首页> 外文期刊>Parasitology >Trichinella spiralis newborn larvae: characterization of a stage specific serine proteinase expression, NBL1, using monoclonal antibodies
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Trichinella spiralis newborn larvae: characterization of a stage specific serine proteinase expression, NBL1, using monoclonal antibodies

机译：旋毛虫新生幼虫：使用单克隆抗体表征阶段特异性丝氨酸蛋白酶表达NBL1

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摘要

Trichinella spiralis is an intracellular parasitic nematode of mammalian skeletal muscle, causing a serious zoonotic disease in humans and showing a high economic impact mainly in pig breeding. Serine proteinases of T. spiralis play important roles in the host-parasite interactions mediating host invasion. In this study, we have focused on newborn larvae (NBL-1), the first identified serine proteinase from the NBL stage of T. spiralis. Five monoclonal antibodies (mAbs) directed against the C-terminal part of NBL1, were produced. These mAbs were IgG1 isotype and specifically recognized as a common motif of 10 amino acids (PSSGSRPTYP). Selected mAbs were further characterized using antigens from various developmental stages of T. spiralis. Western blot revealed that selected mAbs reacted with the native NBL1 at M-r 50 kDa in the adult and NBL mixed antigens and NBL stage alone. Indirect immunofluorescence analysis revealed that selected mAbs intensely stained only the embryos within the gravid females and the NBL. Thus, the produced mAbs are useful tools for the characterization of NBL1 as a major antigen of Trichinella involved in the invasion of the host but also for the development of new serological tests with an early detection of T. spiralis infection.
机译：旋毛虫是哺乳动物骨骼肌的细胞内寄生线虫，在人类中引起严重的人畜共患病，并且主要在猪育种中显示出很高的经济影响。螺旋体丝氨酸蛋白酶在介导宿主入侵的宿主-寄生虫相互作用中起重要作用。在这项研究中，我们专注于新生幼虫（NBL-1），这是最早从螺旋藻NBL阶段鉴定出的丝氨酸蛋白酶。产生了五种针对NBL1 C端的单克隆抗体（mAb）。这些mAb是IgG1同型，被特异性识别为10个氨基酸的常见基序（PSSGSRPTYP）。使用来自螺旋旋毛虫不同发育阶段的抗原进一步表征所选mAb。 Western印迹显示，选定的单克隆抗体在成年小鼠的M-r 50 kDa处与天然NBL1反应，并且仅与NBL混合抗原和NBL阶段发生反应。间接免疫荧光分析显示，所选mAb仅对妊娠雌性和NBL内的胚胎强烈染色。因此，所产生的单克隆抗体是有用的工具，可用于将NBL1表征为旋毛虫的一种主要抗原，该抗原参与宿主的侵袭，同时也可用于开发新的血清学检测方法，以早期发现螺旋螺旋体感染。

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