...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Photochemical & photobiological sciences: the official journal of the European Photochemistry Association and the European Society for Photobiology >Luciferase from Fulgeochlizus bruchi (Coleoptera:Elateridae), a Brazilian click-beetle with a single abdominal lantern: molecular evolution, biological function and comparison with other click-beetle luciferases
【24h】

Luciferase from Fulgeochlizus bruchi (Coleoptera:Elateridae), a Brazilian click-beetle with a single abdominal lantern: molecular evolution, biological function and comparison with other click-beetle luciferases

机译：巴西腹甲虫（Fulgeochlizus bruchi）的萤光素酶（Coleoptera：Elateridae），腹甲有单个腹灯笼：分子进化，生物学功能以及与其他甲虫萤光素酶的比较

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bioluminescent click-beetles emit a wide range of bioluminescence colors (λ_(Max) = 534—594 nm) from thoracic and abdominal lanterns, which are used for courtship. Only the luciferases from Pyrophorus and Pyrearinus species were cloned and sequenced. The Brazilian Fulgeochlizus bruchi click-beetle, which inhabits the Central-west Cerrado (Savannas), is noteworthy because, differently from other click-beetles, the adult stage displays only a functional abdominal lantern, which produces a bright green bioluminescence for sexual attraction purposes, and lacks functional thoracic lanterns. We cloned the cDNA for the abdominal lantern luciferase of this species. Notably, the primary sequence of this luciferase showed slightly higher identity with the green emitting dorsal lantern luciferases of the Pyrophorus genus instead of the abdominal lanterns luciferases. This luciferase displays a blue-shifted spectrum (λ_(Max) = 540 nm), which is pH-insensitive from pH 7.5 to 9.5 and undergoes a slight red shift and broadening above this pH; the lowest K_M for luciferin among studied click-beetle luciferases, and the highest optimum pH (9.0) ever reported for a beetle luciferase. At pH 9.0, the K_M for luciferin increases, showing a decrease of affinity for this substrate, despite the higher activity. The slow luminescence decay rate of F. bruchi luciferase in vitro reaction could be an adaptation of this luciferase for the long and sustained in vivo luminescence display of the click-beetle during the courtship, and could be useful for in vivo intracellular imaging.
机译：生物发光的甲虫从胸灯和腹灯发出各种各样的生物发光颜色（λ_（最大值）= 534-594 nm），用于求偶。仅克隆和测序了来自Pyrophorus和Pyrearinus物种的荧光素酶。值得注意的是，居住在中西部Cerrado（Savannas）的巴西Fulgeochlizus bruchi甲壳虫很重要，因为与其他甲壳虫不同，成年阶段只显示功能性的腹灯，其产生亮绿色的生物发光用于性吸引目的，并且缺少功能性的胸腔灯笼。我们克隆了该物种的腹部灯笼型荧光素酶的cDNA。值得注意的是，该萤光素酶的主要序列与Pyrophorus属的绿色发光背灯笼萤光素酶相比，而不是腹部灯笼虫萤光素酶具有更高的同一性。这种荧光素酶显示出蓝移光谱（λ_（最大值）= 540 nm），该光谱在pH 7.5至9.5范围内对pH值不敏感，并且在此pH值之上略有红移并变宽。在已研究的甲虫萤光素酶中，萤光素的K_M值最低，最佳pH（9.0）也最高。在pH 9.0时，尽管荧光素的活性较高，但荧光素的K_M增加，显示对该底物的亲和力降低。布鲁氏杆菌荧光素酶体外反应的缓慢发光衰减速率可能是这种荧光素酶的适应性，适用于求偶过程中click虫的长期和持续的体内发光显示，并且可用于体内细胞内成像。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号