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Use of STSs and SSRs as rapid and reliable preselection tools in a marker-assisted selection-backcross scheme

机译:STSS和SSRS在标记辅助选择 - Rescross方案中使用STSS和SSRS作为快速可靠的预选工具

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摘要

We describe a new approach for using suitable STS and SSR markers as a powerful molecular tool for screening segregating populations involved in backcross schemes for marker-assisted selection, as a preselection step. Since it can be applied to very large populations, this preselection strategy allows one to increase substantially the pressure of selection at each backcross generation. The technique is fast and reproducible, and can be made even more efficient and costeffective by simultaneous DNA amplification from different primer pairs. In the example illustrated here, three suitable PCR-based markers were used to complete the selection of 300 individuals out of 2300 in less than one month with two people working on the project.
机译:我们描述了一种使用合适的STS和SSR标记作为强大的分子工具,用于筛选回复方案中涉及用于标记辅助选择的分离群体,作为预选步骤。由于它可以应用于非常大的群体,因此这种预选策略允许人们在每个回Cross产生的基本上增加选择的压力。该技术快速且可再现,并且通过来自不同底漆对的同时的DNA扩增,可以更有效和成本效减。在这里所示的示例中,使用三个合适的基于PCR的标记来完成在不到一个月的2300中选择300个个体,并在该项目上工作的两个人在不到一个月内完成。

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