白质软化病,脑室周围

摘要： 目的 运用基于T2WI视觉评估方法评价6～20月龄脑室周围白质软化症(periventricular leukomalacia,PVL)及对照组婴幼儿皮层下白质髓鞘化发育进程,验证该方法筛查性评价婴幼儿皮层下白质髓鞘化发育进程的应用价值.材料与方法 纳入2010年11月至2017年6月间接受磁共振检查的43例PVL及78例MRI未见异常的6～20月龄婴幼儿.PVL分轻、中、重度3组.运用基于常规T2WI视觉评估皮层下白质髓鞘化发育进程的评分方法,评价并获得各组婴幼儿的白质髓鞘化分数.通过Mann-Whitney U检验,比较各组髓鞘化分数;通过线性回归及Spearman相关分析探索PVL各组、对照组全脑及各脑叶皮层下白质髓鞘化分数随龄性变化.P<0.05认为有统计学意义.结果 6～20月龄阶段,PVL轻度组仅顶叶皮层下白质髓鞘化分数与对照有统计学差异(P=0.033);除颞叶外,PVL中度组及重度组的皮层下白质髓鞘化分数与对照组均有差异(P<0.05).Spearman相关分析结果示对照组皮层下白质髓鞘化分数与年龄的相关性高于PVL组.结论 基于T2WI的视觉评估方法在筛查不同程度PVL所致的异常皮层下白质髓鞘化发育进程中,具有良好的可行性.同时,该方法可根据白质髓鞘化发育进程鉴别轻度和中重度PVL.

摘要： 随着产科及新生儿重症监护技术的迅猛发展,具有极限生存活力的早产儿存活率逐年提高.但因神经系统发育不成熟,易受到各种高危因素的影响,致使脑损伤的发病率逐年上升,严重威胁早产儿的生长发育及身心健康.胎盘作为母体与胎儿连接的纽带,近年来已成为研究早产儿神经系统发育不良结局的重要器官.目前研究发现,与早产儿脑损伤有关的胎盘组织病理常伴有急性绒毛膜羊膜炎、胎盘灌注不良、绒毛膜血管病等改变;分子病理可表现为炎症因子释放增加、缺氧诱导因子过度表达与胎盘外泌体保护作用下调等变化;胎盘微小RNA(miRNAs)则可以随着宫内环境的变化而变化,反映出表观遗传调控在保护胎儿神经系统不受外来因素影响中的作用.本文从组织病理学、分子病理学及表观遗传学等方面综述早产儿胎盘的变化特点,及对神经系统发育影响的机制,以及检测早产儿胎盘对评估脑损伤不良结局的意义.

摘要： 目的 以磁共振成像( magnetic resonance imaging ,MRI)资料为依据,对比新生儿生后不同阶段诊断囊性脑室周围白质软化( cystic periventricular leukomalacia ,cPVL)的MRI特点,探讨其与运动发育结局的关系.方法 选取2010年1月至2015年5月本院新生儿科经MRI确诊的cPVL患儿进行回顾性分析,根据确诊时间分为早期诊断组(≤7 d)和晚期诊断组(＞7 d),分析两组患儿cPVL的MRI特点,比较两组患儿门诊随访的运动发育结局.结果 早期诊断组共35例,囊腔主要位于侧脑室前角(35例),其次为侧脑室体部(2例)和半卵圆中心(1例),囊腔数量单个17例,2个14例,3 个及以上4 例;晚期诊断组共45 例,囊腔主要位于半卵圆中心(35 例)和侧脑室后角(34例),其次为侧脑室体部(20例)和侧脑室前角(10例),囊腔数量单个3例,2个5例,3个及以上37例.早期诊断组共随访23例,22例肢体运动正常,1例出现痉挛性双瘫(4.3%);晚期诊断组共随访24例,4例肢体运动正常,20例出现痉挛性偏瘫、双瘫或四肢瘫(83.3%),晚期诊断组脑性瘫痪发生率高于早期诊断组,差异有统计学意义( P＜0.05).结论 生后不同时期诊断cPVL患儿的MRI所示囊腔位置、数量均有不同,早期被证实存在cPVL 的患儿运动发育结局较好,提示宫内发生的cPVL预后优于生后获得的cPVL.%Objective To compare the magnetic resonance imagings (MRI) of neonates diagnosed with cystic periventricular leukomalacia (cPVL) at different stages after birth , and to clarify the relationship of MRI and motor development outcomes.Method Data of neonates admitted to the Shengjing Hospital of China Medical Univerisity from January 2010 to May 2015 diagnosed with cPVL by MRI were studied retrospectively.Subjects were assigned into two groups according to time of diagnosis : early－diagnosed group (≤7 d) and late－diagnosed group (＞7 d).The MRI and subsequent motor development outcome were compared between two groups.Result There were 35 neonates in early－diagnosed group.The cysts were mainly located in the anterior horn of the lateral ventricle (35 infants), the body of the lateral ventricle (2 infants) and the centrum semiovale (1 infants).Only one cyst were found in 17 infants, two cysts in 14 infants, three or more cysts in 4 infants.There were 45 cases in the late－diagnosed group, the cysts were mainly located in the centrum semiovale ( 35 infants ) and the posterior horn of the lateral ventricle (34 infants), the body (20 infants) and the anterior horn (10 infants) of lateral ventricle.Only one cyst were found in 3 infants, two cysts in 5 infants, three or more cysts in 37 infants.Among the 23 infants in the early－diagnosed group with follow－up, 22 infants are clinically normal , one infant with spastic diplegia (4.3%).Among the 24 infants in the late－diagnosed group with followe－up, 4 infants are clinically normal , 20 infants with spastic hemiplegia , diplegia or quadriplegia (83.3%).There are significant differences of incidence of cerebral palsy between the two group (P＜0.05).Conclusion MRI imaging showed that the location, number of cysts are different between the early－diagnosed and late－diagnosed group, and the motor development outcome of the early－diagnosed group are better , which indicates the prognosis of cPVL that occurred in utero are better than acquired cPVL after birth.

摘要： Objective To explore the mechanisms of vascular endothelial growth factor receptor 2 (VEGFR2) expression regulated by recombinant human erythropoietin (rh-EPO) in a premature rat model of periventricular white matter damage.Methods Sprague-Dawley rats aged three days were randomly divided into five groups:sham group without hypoxia-ischemia (HI),HI group (HI with saline administration),HI+erythropoietin (EPO) group,HI+erythropoietin receptor (EPOR) antagonist group and HI+EPO+EPOR antagonist group.Rat pups were either subjected to permanent ligation of the right common carotid artery and 6％ O2+94％ N2 for two hours (HI) or sham operated and exposed to normal air (sham).After the operation,rats in the HI+EPOR antagonist and HI+EPO+EPOR antagonist groups received a single intraventricular injection of EPOR antagonist (5 μ l).Four hours after the operation,rats in the HI+EPO and HI+EPO+EPOR antagonist groups received a single intraperitoneal injection of rh-EPO (5 U/g).Western-blot was performed to detect EPOR,phosphorylated EPOR (p-EPOR),extracellular regulated protein kinases (ERK) and phosphorylated ERK (p-ERK) at 60 and 90 minutes after the models were established successfully,and also used to analyze the expression of VECFR2 on day 2 and 4.Analysis of variance and SNK test were used as statistical methods.Results At 60 and 90 minutes after model establishment,the expression of EPOR protein in rat brain tissues was increased in HI (1.717±0.206 and 1.416±0.242),HI+EPO (2.557±0.222 and 2.111±0.159) and HI+EPO+EPOR antagonist (1.547±0.170 and 1.452±0.250) groups as compared with that in sham group (1.095±0.182 and 0.751 ±0.136),that in HI+EPO group was higher than that in HI and HI+EPO+EPOR antagonist groups,and that in HI+EPOR antagonist group (1.088±0.160 and 1.020±0.174) was lower than that in HI group.All differences were statistically significant (F=30.154 and 20.265,both P＜0.05).The expressions of p-EPOR,p-ERK and VEGFR2 in the five groups were consistent with the expression of EPOR,and the differences were also statistically significant (all P＜0.05).In addition,the expression of VEGFR2 in HI+EPO+EPOR antagonist group was lower than that in HI group on day 4 (1.053 ± 0.118 vs 1.439± 0.074,F=54.248,P＜0.05).No statistically significant difference in ERK expression was found among all groups at 60 or 90 minutes after modeling (F=1.117 and 0.734,both P＞0.05).Conclusions ERK signaling pathways will be affected by EPO binding to EPOR.As a result,VEGFR2 expression was increased leading to enhanced angiogenesis in a premature rat model of periventricular white matter damage.%目的 探讨在早产儿脑白质损伤模型鼠脑血管生成中,重组人促红细胞生成素(recombinant human erythropoietin,rh-EPO)调控血管内皮生长因子受体2(vascular endothelial growth factor receptor 2,VEGFR2)表达的可能机制. 方法 选取3日龄Sprague-Dawley仔鼠,采用随机数余数分组法分为假手术组、缺血缺氧组、缺血缺氧+促红细胞生成素(erythropoietin,EPO)组、缺血缺氧+EPO受体(erythropoietin receptor,EPOR)拮抗剂组、缺血缺氧+EPO+EPOR拮抗剂组.假手术组仔鼠仅游离右侧颈总动脉.缺血缺氧组仔鼠结扎右侧颈总动脉,并吸入氮氧混合气体2h.缺血缺氧+EPO组、缺血缺氧+EPO+EPOR拮抗剂组结扎后4h腹腔内给予rh-EPO 5 U/g.缺血缺氧+EPOR拮抗剂组、缺血缺氧+EPO+EPOR拮抗剂组,结扎后脑室内给予EPOR拮抗剂5μl,其余组均给予等量生理盐水.采用蛋白质印迹技术检测分组处理完成后60和90 min脑组织中EPOR和磷酸化EPOR(phosphorylated-EPOR,p-EPOR)、总细胞外信号调节激酶(extracellular regulated protein kinases,ERK)和磷酸化ERK(phosphorylated-ERK,p-ERK)及分组处理完成后2、4d脑组织中的VEGFR2表达情况.多组间数据比较采用单因素方差分析,组间两两比较采用SNK检验. 结果 分组处理完成后60和90 min,仔鼠脑组织EPOR蛋白表达在假手术组呈较低水平(分别为1.095±0.182和0.751±0.136);在缺血缺氧组(1.717±0.206和1.416±0.242)、缺血缺氧+EPO组(2.557±0.222和2.111±0.159)和缺血缺氧+EPO+EPOR拮抗剂组(1.547±0.170和1.452±0.250)表达增加,高于假手术组;在缺血缺氧+EPO组高于缺血缺氧组和缺血缺氧+EPO+EPOR拈抗剂组;在缺血缺氧+EPOR拮抗剂组(1.088±0.160和1.020±0.174)低于缺血缺氧组;差异均有统计学意义(F值分别为30.154和20.265,P值均＜0.05).p-EPOR和p-ERK,以及分组处理完成后2和4 d VEGFR2在各组的表达差异与EPOR一致,差异均有统计学意义(P值均＜0.05);此外,分组处理完成后4d,VEGFR2表达在缺血缺氧+EPO+EPOR拮抗剂组低于缺血缺氧组(1.053±0.118与1.439±0.074,F=54.248,P＜0.05).分组处理完成后60和90 min,各组仔鼠脑组织ERK表达差异均无统计学意义(F值分别为1.117和0.734,p值均＞0.05). 结论 在早产儿脑白质损伤模型鼠,EPO与EPOR作用后影响下游ERK信号通路,增加脑组织VEGFR2的表达.

摘要： 目的 探讨新生儿脑室周围白质软化症(periventricular leucomalacia,PVL)发生的高危因素.方法 选取2007年1月-2015年12月入住解放军白求恩国际和平医院新生儿科并经床旁颅脑超声诊断为PVL的62例新生儿作为PVL组,另选取同期无PVL的76例新生儿作为无PVL组,回顾性分析两组围生期临床资料,采用单因素和多因素分析寻找PVL的高危因素,并观察两组预后.结果 单因素分析显示:双胎、宫内缺氧史、产前感染、应用机械通气、机械通气持续时间、C-反应蛋白异常、白细胞异常、1 min Apgar评分、合并低碳酸血症、合并重度颅内出血、合并感染、合并肺炎、合并呼吸窘迫综合征、合并窒息及窒息持续时间,两组比较差异均有统计学意义(P ＜0.05或P＜0.01).多因素logistic回归分析显示:合并低碳酸血症、合并感染、机械通气持续时间是PVL的高危因素.PVL组后遗症发生率和病死率均高于无PVL组,差异均有统计学意义(P均＜0.01).结论 合并低碳酸血症、合并感染、机械通气持续时间是新生儿PVL的危险因素,给予围生期积极有效的预防措施,可减少新生儿PVL的发生.

摘要： 目的 探讨脑室周-脑室内出血(PVH-IVH)合并脑白质软化(PVL)的早产儿甲状腺激素水平的变化.方法 选取2015年8月至2016年12月入住该院早产儿病区及新生儿重症病房的胎龄32～34周的适于胎龄儿92例作为研究对象.出生后4周依据头颅磁共振成像(MRI)结果是否合并PVL将其分为PVL组和非PVL组.所有研究对象分别于出生后1、4周清晨9:00采集外周血,采用化学发光法测定血清游离三碘甲腺原氨酸(FT3)、血清游离甲状腺素(FT4)、促甲状腺激素(TSH)值,对比分析不同组治疗前后及组间FT3、FT4、TSH的水平变化.结果 PVL组4周时FT3、FT4水平均较非PVL组低,差异均有统计学意义(P<0.05),TSH水平高于非PVL组,差异有统计学意义(P<0.05),PVL组出生后1周时FT3、FT4水平均低于非PVL组,差异均有统计学意义(P<0.05),TSH水平高于非PVL组,差异有统计学意义(P<0.05).结论 早产儿PVH-IVH是否好转及是否合并PVL与低甲状腺功能水平相关,提示动态检测PVH-IVH早产儿甲状腺激素水平对判断和评估预后有一定的临床意义.

摘要： Objective To study inflammatory cytokines changes in brain tissue of neonatal rats with periventricular leukomalacia (PVL).Method A total of 80 neonatal SD rats (P3) were randomly assigned into 2 equal groups,sham-operated group and PVL group.Rats in each group were further assigned into four subgroups (12,24,48,72 h),with 10 rats in each subgroup.The hypoxic-ischemic PVL modal were established following the procedure:first,isolation and ligation of left common carotid artery,and then exposed to 8％ O2 and 92％ N2 for 2.5 h.The sham-operated rats were processed with isolation of left common carotid artery only.Rats of the four subgroups were sacrificed at 12,24,48 h and 72 h respectively,then the brains were rapidly removed in corresponding time.Pathological changes of brain tissues were observed using HE stain.The mRNA expression levels of tumor necrosis factor α(TNF-α)and inter leukin-1β (IL-1β) were assessed using real-time quantitative PCR assays,the protein levels of TNF-α and IL-1β were detected using enzyme linked immunosorbent assay method.Result The brains tissues of rats in PVL group showed remarkably hyperemia and edema,with left ventricle enlargement.Periventricular white matter structure was disintegrated comparing with sham-operated group.The expression of TNF-α and IL-1 β mRNA in PVL group increased significantly,reaching peak by 24 h and then gradually decreased 72 h after the procedure.The mRNA levels of TNF-c and IL-1 β were significantly different between each two time points of 12,24 h and 48 h in PVL group (P ＜0.05).However,there was no differences between 72 h and 48 h within PVL and sham-operated group group(P ＞0.05).In PVL group,the protein expression trends of TNF-α and IL-1 β were similar to mRNA expression trends.Moreover,the protein levels were significantly different between each two time points of TNF-α and IL-1 β,respectively (P ＜ 0.01).The protein expression levels of TNF-α were different at each time point between PVL group and sham-operated group[(189.2 ± 20.4) pg/ml vs.(131.4 ±5.2) pg/ml at 12 h,(213.8 ± 16.7) pg/ml vs.(127.7 ±7.4) pg/ml at 24 h,(181.7 ± 15.0) pg/ml vs.(126.3 ± 6.0) pg/ml at 48 h,(159.6 ± 25.3) pg/ml vs.(131.4 ± 6.0) pg/ml at 72 h;P ＜0.01].The protein levels of IL-1β were different between the two groups only at 24 h and 48 h.[(121.8 ±30.0) pg/ml vs.(67.4 ± 13.7) pg/ml,(83.3 ± 15.7) pg/ml vs.(65.3 ± 14.9) pg/ml;P ＜0.05].In sham-operated group,no differences of TNF-α and IL-1 β protein levels were found between any different time points (P ＞ 0.05).Conclusion Inflammatory cytokines such as TNF-α and IL-1β are involved in ischemic-hypoxia induced PVL.Dynamic detection of inflammatory factors is expected to be an important method of early diagnosis,assessment of treatment efficacy and prognosis of PVL.%目的 探讨脑室周围白质软化(PVL)新生大鼠的炎症损伤情况.方法 选择3日龄SD大鼠80只,随机分为假手术组和PVL组各40只,每组又分为12、24、48、72 h4个亚组各10只.PVL组通过单侧颈总动脉结扎及术后吸入8％氧气+92％氮气混合气2.5h制备缺血缺氧型PVL新生大鼠动物模型;假手术组仅游离左侧颈总动脉,不予结扎及缺氧.两组大鼠于4个时间点分别断头取脑组织,应用苏木精-伊红(HE)染色观察脑组织病理改变;实时荧光定量逆转录-聚合酶链反应(RT-qPCR)法检测肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β) mRNA表达水平;酶联免疫吸附试验(ELISA)法检测TNF-α及IL-1β3蛋白含量.结果 HE染色可见PVL组大鼠脑组织较假手术组明显充血、水肿,左侧脑室扩大,脑室周围白质结构稀疏、紊乱.PVL组TNF-α及IL-1β mRNA表达上调,均出现随时间推移先升高,24h达高峰,随后逐渐降低的趋势,且12、24、48 h两两比较,差异均有统计学意义(P ＜0.05);72 h后TNF-α及IL-1β mRNA表达水平均下调,与同组48 h相比差异无统计学意义(P＞0.05).PVL组各时间点TNF-α及IL-1β蛋白含量比较差异有统计学意义(P＜0.01),且各时间点TNF-α蛋白含量均高于假手术组[12 h(189.2 ±20.4) pg/ml比(131.4 ±5.2) pg/ml、24h(213.8±16.7)pg/ml比(127.7±7.4) pg/ml、48 h(181.7±15.0)pg/ml比(126.3±6.0)pg/ml、72 h(159.6±25.3)pg/ml比(131.4±6.0) pg/ml,P＜0.01];IL-1β蛋白含量仅在24、48 h与假手术组相比差异有统计学意义[(121.8±30.0) pg/ml比(67.4±13.7) pg/ml、(83.3±15.7) pg/ml比(65.3±14.9)pg/ml,P＜0.05)].假手术组TNF-α及IL-1β蛋白含量各时间点比较,差异无统计学意义(P＞0.05).结论 TNF-α与IL-1β共同参与缺血缺氧诱导的PVL,且具有先升高后降低的时间趋势,动态监测其变化有望成为PVL早期诊断、疗效观察、预后及评估的重要手段.

摘要： 目的:应用彩色多普勒超声与Snake模型图像分析软件,探讨超声灰度值定量分析对早产儿脑室周围白质软化(Periventricular leukomalacia,PVL)诊断的价值.方法:对生后7d内临床诊断为PVL的早产儿120例及正常新生儿对照组80例进行常规颅脑超声检查,根据超声图像的灰度解剖分布,利用Snake模型图像分析软件自动提取实验感兴趣区,用“手动分析”进行边缘修正,从而提取被确定的5个感兴趣区(ROIs):基底节区、侧脑室前角旁脑白质、侧脑室后角旁脑白质、脉络丛和小脑蚓部.每个ROI标准切面连续录2幅图像进行数字化存储,用于离线分析,将每个相应结构的ROI描绘出来,并计算其平均的超声灰度值.对120例PVL早产儿实验数据进行可重复性(图像分析软件变异,EV)及可再现性(测量者变异,AV)的分析,用95％可信区间来反映其变异程度.结果:PVL患儿基底节区、侧脑室前角旁脑白质及侧脑室后角旁脑白质平均灰度值分别为130.64±4.12、131.35±3.02、133.46±2.94,高于对照组(92.51±6.89、81.64±2.78、85.75±3.65),差异有统计学意义(μ=44.49、119.78、97.57,P＜0.01);PVL患儿脉络丛、小脑蚓部平均灰度值分别为132.90±4.88、132.25±2.56,与对照组(131.98±5.82、131.43±4.47)无显著性差异.PVL患儿重复性EV为2.70％,再现性AV为0.56％,总的重复性及再现性变异(Repeatability and reproducibility,R&R)为2.70％.结论:Snake模型脑组织灰度值定量分析评估,为临床早期诊断早产儿脑室周围白质软化提供了可靠的方法.

摘要： 目的:探讨脑室周围白质软化症临床CT特征.方法:对83例脑室周围白质软化症患者临床CT资料进行回顾性分析.结果:脑室旁软化灶型14例,其中位于双侧枕角旁5例;右侧脑室体部及前、后角旁各2例;左侧脑室体旁3例,枕角旁2例,同时伴有软化灶旁白质减少和皮层灰质变薄;弥漫性白质减少型69例,其中发生于枕角旁39例,前、后角旁15例,弥漫性白质减少15例;合并脱髓鞘病灶7例,胼胝体发育不良19例.CT表现为脑室旁见斑点状或条索状软化灶,个别病例呈球囊状改变;双侧脑室前、后角旁白质容量减少,重者半卵圆中心残留少量白质,枕角呈直角状扩大,边缘僵直,灰质层逼近脑室外侧缘.统计结果表明不同孕期胎龄生产史对病变类型的分布有差异(x2=52.28,P＜0.05),弥漫性白质减少分布部位与孕期胎龄生产史间有差异(x2-25.89,P＜0.05).围产期脑白质损伤严重程度与胎龄有相关,孕期胎龄愈小,脑白质损伤愈严重.结论:脑室周围白质软化症具有特征性,CT检查能够清楚地显示脑白质损伤的病理解剖形态.

摘要： 目的 应用扩散张量成像(diffusion tensor imaging,DTI)及扩散纤维束成像(diffusion tensor tractography,DTT)评价轻度脑室旁脑白质软化症(perventricular leukomalacia,PVL)患儿脑白质的损伤,探讨DTI及DTT对轻度脑室旁白质软化症的诊断价值.方法 应用GE Signa Excite 1.5 T磁共振扫描仪对25例PVL患儿和月龄及性别匹配的25例健康对照组分别进行头颅MRI平扫及DTI检查.测量2组患儿胼胝体膝部、胼胝体压部、双侧内囊前肢、双侧内囊后肢、视放射、大脑脚及上纵束的各向异性分数(fractional anisotropy,FA)值及平均扩散系数(average diffusion coffient,ADC)值.对2组感兴趣测量结果进行独立样本t检验,分析2组间FA值及ADC值有无差异,分析有差异部位与PVL患儿临床症状的关系.对2组患儿行扩散张量纤维束成像,对比观察两组患儿纤维束的走行及分布.结果 PVL组患儿胼胝体压部、视放射及上纵束FA值显著低于正常对照组(P＜0.05或＜0.01);PVL组胼胝体压部及上纵束ADC值显著高于正常对照组(P＜0.05或＜0.01).扩散张量纤维束成像显示:PVL组患儿胼胝体压部周围的纤维束较正常对照组稀疏、中断、紊乱.结论 轻度PVL患儿的脑白质损伤多发生于胼胝体压部、视放射及上纵束,是其合并双下肢痉挛性瘫痪、视力损害、精神运动发育迟缓的主要原因.DTI及DTT可以评价PVL患儿脑白质损伤的范围及程度,为临床治疗及随访提供依据.

摘要： 本发明涉及阿戈美拉汀在制备用于治疗脑室周围白质软化的药物中的用途。

摘要： 本发明提供了一种高软化点沥青的制备方法，包括如下步骤：将改质沥青导入单体容器，向所述单体容器内通入惰性气体，所述单体容器上下依次设置有至少三段各自独立的加热区域，每段加热区域内设置有独立的加热器，且加热温度依次递增，反应压力为0.03～0.05MPa；所述改质沥青加热达到目标温度后，保持温度6～18h，得到所述高软化点沥青。同时提供了所用的制备装置、制备的高软化点沥青，以及利用高软化点沥青制备的粘结剂。本发明能够将改质沥青的软化点控制在一个较窄的范围内，使之质量更加稳定，从而能够制备出具有高软化点、高固定碳素含量的高软化点沥青以及粘结剂。

摘要： 本发明涉及使用直接质谱分析测量血液样品中非糖化和糖化血红蛋白水平的方法。可使用糖化血红蛋白分子的比例来诊断前驱糖尿病或糖尿病。

摘要： 本发明涉及阿戈美拉汀在制备用于治疗脑室周围白质软化的药物中的用途。

摘要： 本发明属于生物技术领域，尤其涉及实时荧光PCR检测家蚕传染性软化病病毒的方法及其试剂盒、引物和探针。上游引物的序列为GGGAAAGTCGATGAATGCCC，下游引物的序列为AAGTGTGCCCAGSTATARCT，下游引物中S随机采用G或C替代，R随机采用A或G替代，所述的探针为FAM- TTGAGGAGAACGCCAAGGAA –Eclipse。本发明由于引物和探针具有很高的特异性和灵敏度，提高了早期检测的检出率，并简化了检测方法。本发明能提高家蚕中上述病原物的早期检测的检出率，可有效的防止疾病的传播，减少经济损失。

摘要： 本发明提供一种选择性杀灭含有蛋白质聚集体的细胞的方法、其试剂盒、蛋白质错误折叠病治疗药及自血液制品中去除蛋白质聚集体的制剂。在一实施方案的选择性杀灭细胞的方法中，使作为检测蛋白质聚集体的化合物的含有氟代醇或由下述通式(1)表示的化合物的分子结构修饰剂作用于细胞，从而选择性杀灭含有蛋白质聚集体的细胞。通式(1)中，a为0或1，当a＝1时，R1为氢原子且R2为羟基、或者R1为羟基且R2为氢原子，当a＝0时，R2为与碳原子形成双键的氧原子，R3为CHlClmFn，l、m及n为1～3的整数且满足l+m+n＝3，R4为CHsCltFu，s、t及u为1～3的整数且满足s+t+u＝3。

摘要： 本发明涉及通过给药含有化学式1的氨基甲酸酯化合物的药物组合物而用于预防、缓解或治疗糖尿病性周围神经病变或化疗诱发性周围神经病变的用途。

摘要： 本发明公开一种光软化性树脂组合物，其含有：具有双硫键的化合物；及自由基捕获剂。并且，本发明公开一种固化性树脂组合物，其含有：具有双硫键且具有官能基的单体；具有能够与官能基进行反应的取代基的单体；及自由基捕获剂。

摘要： 本发明提供了一种高软化点沥青的制备方法，包括如下步骤：将改质沥青导入单体容器，向所述单体容器内通入惰性气体，所述单体容器上下依次设置有至少三段各自独立的加热区域，每段加热区域内设置有独立的加热器，且加热温度依次递增，反应压力为0.03～0.05MPa；所述改质沥青加热达到目标温度后，保持温度6～18h，得到所述高软化点沥青。同时提供了所用的制备装置、制备的高软化点沥青，以及利用高软化点沥青制备的粘结剂。本发明能够将改质沥青的软化点控制在一个较窄的范围内，使之质量更加稳定，从而能够制备出具有高软化点、高固定碳素含量的高软化点沥青以及粘结剂。

摘要： 本实用新型是一种用于饮用水软化的可控软化和可软化料再生的硬水软化装置。它包含有钠离子树脂的软化室，软化室的硬水进水管上有通过三通接头连接一根硬水管，其上有硬水控制阀，软化室的软水管上有软水控制阀，一个三通接头连通硬水管、软水管和软水出水管。通过硬水控制阀和软水控制阀可控制混合后的软化水硬度指标。在软化室的软水管至软化室底部出水口间连接一个电动泵，用作软化料再生时驱动软化料再生剂循环，可对软化料进行再生。