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细胞化学

细胞化学的相关文献在1983年到2023年内共计523篇,主要集中在基础医学、肿瘤学、中国医学 等领域,其中期刊论文469篇、会议论文5篇、专利文献208557篇;相关期刊253种,包括水生生物学报、分子细胞生物学报(英文版)、植物科学学报等; 相关会议5种,包括中国南方十六省(市、区)水产学会渔业学术论坛暨第二十六次学术交流大会、第二届全国医学系统、第八届全国农林系统电子显微镜学术交流会、第十一次全国电子显微学会议等;细胞化学的相关文献由1084位作者贡献,包括吴正治、康振生、郭振球等。

细胞化学—发文量

期刊论文>

论文:469 占比:0.22%

会议论文>

论文:5 占比:0.00%

专利文献>

论文:208557 占比:99.77%

总计:209031篇

细胞化学—发文趋势图

细胞化学

-研究学者

  • 吴正治
  • 康振生
  • 郭振球
  • 刘平
  • 徐根兴
  • 田国伟
  • 谢锦玉
  • 陈万新
  • 陈文列
  • 周小青
  • 期刊论文
  • 会议论文
  • 专利文献

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    • 郑瑞; 章鸯; 陈葆国; 沈芝颖; 林爱芬
    • 摘要: 目的 比较免疫组化、细胞化学及流式细胞学3种方法检测急性髓系白血病(AML)胞内MPO阳性率的差异及临床意义.方法 对90例AML患者同时进行骨髓活检组织免疫组织化学染色,细胞涂片细胞化学染色及骨髓流式细胞学分析,检测MPO表达情况.结果 3种方法联合检测,AML患者MPO总阳性率为95.5%,其中免疫组织化学、细胞化学及流式细胞学方法检测MPO阳性率分别为66.7%、93.3%及87.8%,以免疫组化方法检测MPO敏感度最低,明显低于细胞化学和流式细胞学检测方法(P均0.05);AML患者中,除M7外,以M5患者MPO阳性率最低(88.9%),其中免疫组织化学法MPO的检出率最低(37.0%),明显低于细胞化学法(88.9%,P0.05).结论 细胞化学及流式细胞术检测AML患者MPO表达的阳性率优于活检组织的免疫组化法,三者结合比单独一种更有利于AML与急性淋巴细胞白血病的鉴别诊断.
    • CHEN Ting-Ting; REN Qiu-Nan; CHEN Xian-Xian; CAO Xin-Fang; HUANG Hui-Hui; ZHANG Sheng-Zhou
    • 摘要: 采用常规瑞氏染色和细胞化学染色方法对团头鲂(Megalobrama amblycephala)外周血细胞的显微结构及细胞化学特征进行了观察.在团头鲂外周血细胞中可区分出六类细胞:红细胞、淋巴细胞、单核细胞、嗜中性粒细胞、嗜酸性粒细胞和血栓细胞.其中淋巴细胞是除红细胞外含量最多的细胞,其次分别为血栓细胞、单核细胞、嗜中性粒细胞、嗜酸性粒细胞.成熟红细胞多为卵圆形,表面光滑,胞核呈椭圆形或圆形,染色质较为致密;淋巴细胞多呈圆形,胞质较少,胞核常偏位;单核细胞多为圆形,胞核呈圆形或椭圆形,胞质内可见空泡状结构;嗜中性粒细胞近似圆形,胞核常偏于细胞一侧,呈分叶状、肾形或椭圆形,核质界限清晰;嗜酸性粒细胞一般为圆形,胞核为肾形或椭圆形,胞质中充满紫红色颗粒;血栓细胞形态多样,主要有椭圆形、纺锤形、长杆状和泪滴形,核质比较大.淋巴细胞呈α-醋酸萘酚酯酶(ANAE)阳性,呈过碘酸-雪夫(PAS)、氯乙酸AS-D萘酚酯酶(AS-DCE)弱阳性,呈苏丹黑B(SBB)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)及过氧化物酶(POX)阴性;单核细胞呈POX、ACP强阳性,PAS、SBB、AS-DCE和ANAE为阳性,呈AKP阴性;嗜中性粒细胞除PAS和ANAE为弱阳性外,其他染色结果和单核细胞相同;嗜酸性粒细胞呈POX、ANAE强阳性,SBB、ACP阳性,PAS及AS-DCE则为弱阳性,呈AKP阴性;血栓细胞呈PAS、AS-DCE及ANAE弱阳性,呈SBB、ACP、AKP及POX阴性.团头鲂外周血细胞的显微结构及细胞化学特征与其他鱼类具有相似之处,但亦有其明显的物种特异性.该研究结果可作为监测团头鲂健康状态的依据,为其养殖及病理诊断提供基础资料.
    • 张辉霞
    • 摘要: 现如今,社会发展愈发完善,人们的生活水平日益富足,促使食品安全受到更多的重视与关注,食品安全与食品贸易的联系日益紧密,这也对我国食品安全也提出新的要求。为了保障食品安全与食品贸易平稳发展,减少或者避免细菌、真菌等微生物对食品安全造成的不良影响,需要提高食品检测力度。为此,流式细胞数等快速检验方法被普遍运用到微生物快速检测领域中,而流式细胞术将光学、电子学、流体力学、细胞化学、免疫学、激光以及计算机等融合为一体,是先进的技术性方法之一。故此,将进一步分析流式细胞术在微生物快速检测领域的机制,以期提升我国食品安全水平。
    • 姚瑞玲; 李慧娟; 张晓宁; 王胤
    • 摘要: 马尾松(Pinus massoniana)是我国南方生态建设与造林用材的主要树种,为了揭示马尾松抗虫机理尤其是诱导抗虫性的分子机制,该研究以马尾松幼苗为材料,通过外源喷施茉利酸甲酯(MeJA),分析了处理与对照间植株针叶显微结构、萜类合成酶活性及其细胞化学定位的变化.结果表明:在0.2 mmol·L-1 MeJA处理下马尾松植株松针中萜类物质,尤其是单萜、二萜的相对含量增加,马尾松毛虫拒食性明显,诱导抗性增强.显微观测中,针叶叶肉细胞内树脂道分泌物增加,叶绿体数目减少,但叶绿体体积增大,叶绿体片层结构增加.MeJA处理4周后,针叶中萜类合成酶活性增加,通过电镜酶细胞化学观察,膜系统尤其是叶绿体膜上萜类合成酶活性定位明显增强.这说明MeJA诱导的马尾松诱导抗性可能与改变的叶绿体结构及绿色质体萜类合成酶活性密切相关.
    • 戴锡玲; 王赛赛; 曹建国; 王全喜
    • 摘要: 采用透射电镜和细胞化学技术对红盖鳞毛蕨(Dryopteris erythrosora(Eaton)O.Ktze.)的孢子发育过程进行了研究,根据超微结构和细胞化学特征可将其孢子发育过程分为3个阶段:(1)孢子母细胞及其减数分裂阶段:孢子母细胞壳在孢原细胞末期开始形成,位于孢子母细胞及其减数分裂形成的四分体外侧,PAS反应显示其为多糖性质,与胼胝质壁为同功结构;在减数分裂形成的四分孢子之间产生孢子外壳,从功能、形成位置和时间上看与胼胝质壁相似,但苏丹黑B反应显示其可能含有脂类物质,与孢子母细胞壳和胼胝质壁不同。(2)孢子外壁形成阶段:外壁为乌毛蕨型(Blechnoidal-type),由薄的多糖性质的外壁内层和表面平滑的孢粉素外壁外层构成;小球参与外壁外层的形成,组织化学分析显示小球的中央区域和外壁外层内侧部分由红色(多糖)变为黄色,小球的表面区域和外壁外层部分始终被染成黑色(脂类),可知小球与外壁同步发育。(3)孢子周壁形成阶段:周壁为凹陷型(Cavate-type),包括2层,内层薄,紧贴外壁,外层隆起形成孢子脊状褶皱纹饰的轮廓,以少见的向心方向发育;苏丹黑B和PAS反应观察周壁被染成橙色,推测其可能由多糖等成分构成;孢子囊壁细胞参与周壁的形成。本研究为揭示蕨类植物孢子发生的细胞学机制提供了新资料。
    • 戴锡玲; 王赛赛; 曹建国; 王全喜
    • 摘要: 采用透射电镜和细胞化学技术对红盖鳞毛蕨(Dryopteris erythrosora (Eaton)O.Ktze.)的孢子发育过程进行了研究,根据超微结构和细胞化学特征可将其孢子发育过程分为3个阶段:(1)孢子母细胞及其减数分裂阶段:孢子母细胞壳在孢原细胞末期开始形成,位于孢子母细胞及其减数分裂形成的四分体外侧,PAS反应显示其为多糖性质,与胼胝质壁为同功结构;在减数分裂形成的四分孢子之间产生孢子外壳,从功能、形成位置和时间上看与胼胝质壁相似,但苏丹黑B反应显示其可能含有脂类物质,与孢子母细胞壳和胼胝质壁不同.(2)孢子外壁形成阶段:外壁为乌毛蕨型(Blechnoidal-type),由薄的多糖性质的外壁内层和表面平滑的孢粉素外壁外层构成;小球参与外壁外层的形成,组织化学分析显示小球的中央区域和外壁外层内侧部分由红色(多糖)变为黄色,小球的表面区域和外壁外层部分始终被染成黑色(脂类),可知小球与外壁同步发育.(3)孢子周壁形成阶段:周壁为凹陷型(Cavate-type),包括2层,内层薄,紧贴外壁,外层隆起形成孢子脊状褶皱纹饰的轮廓,以少见的向心方向发育;苏丹黑B和PAS反应观察周壁被染成橙色,推测其可能由多糖等成分构成;孢子囊壁细胞参与周壁的形成.本研究为揭示蕨类植物孢子发生的细胞学机制提供了新资料.%Spore development of Dryopteris erythrosora (Eaton) O.Ktze.was studied by transmission electron microscopy and cytochemical technology.Based on ultrastructural and cytochemical features,spore development was divided into three stages.(1) Spore mother cell and meiosis stage:sporocyte coat is formed at the late stage of the archesporial cells.The sporocyte coat covers the spore mother cells and the outer surface of the tetrads.The PAS reaction shows that this coat is polysaccharide in nature.It is an analogous structure with callose.During meiosis,spore coats are formed between the tetrad spores.The spore coats resemble the callose wall in function,formation site,and formation time.However,Sudan Black B staining shows that the spore coats may contain lipid material,which does not exist in the callose wall.(2) Exospore formation stage:exospore formation is Blechnoidal-type.The exospore consists of two layers,i.e.thin inner exospore and thick outer exospore.The former is composed of polysaccharides and the latter is composed of sporopollenin with a smooth outer surface.Globules participate in the formation of the outer exospore.Cytochemical staining shows that the center of the globules and inner part of the outer layer of the exospore are yellow,but the outer part of the globules and outer layer of the exospore became black when stained (probably containing lipids).It can be inferred that the globules and exospore develop simultaneously.(3) Perispore formation stage:perispore formation is Cavete-type.The perispore consists of an inner perispore and outer perispore.The thin inner perispore appresses the exospore closely.The outer perispore projects outwards and forms the outline of the ridge ornamentation of the spore.The development of the perispore is centripetal.Sudan Black and PAS reaction stain the perispore orange,indicating that the perispore may be composed of several polysaccharides.Spore wall cells participate in formation of the perispore.The present investigation provides new data for sporogenesis and spore wall development,which will contribute to revealing the cytological mechanism of sporogenesis.
    • 赵柳兰; 代敏; 杨淞; 吴应斌; 汤巨平; 杨世勇; 杜宗君
    • 摘要: 通过对成年似鲇高原鳅(Triplophysa siluroides)外周血细胞涂片及中肾、肝脏和脾脏组织的印片进行瑞氏(Wright's)、过碘酸雪夫氏(PAS)和苏丹黑B(SBB)染色,观察各类血细胞形态、大小、分布及细胞化学特征.结果表明:似鲇高原鳅血细胞可分为红细胞、粒细胞、单核细胞、淋巴细胞和血栓细胞,各类血细胞的发生(除血栓细胞)均可分为原始、幼稚和成熟三个阶段.外周血中红细胞比例最多,为97.56%,白细胞中血栓细胞数量最多占52.86%,居白细胞的首位;三种粒细胞中嗜中性粒细胞数量最多占30.25%.粒细胞的主要发生场所在中肾占45.18%,红细胞的主要发生场所在脾脏,占71.62%,肝脏中未见原始血细胞.在中肾中同时可见嗜酸性、嗜碱性和嗜中性三种粒细胞的发生.各阶段的白细胞PAS染色均显示阳性,但只有各阶段的粒细胞SBB染色显示阳性.%The methods of Wright's,Periodic acid-Schiff (PAS)and Sudan back B (SBB)staining were used to dye the peripheral blood cells and the kidney, spleen and liver in Triplophysa siluroides to observe the morphology, size, distribu-tion and cytochemical characteristics of various blood cells.The results showed that the blood cells could bedevided into 5 types as erythrocytes, granulocytes, monocytes, lymphocytes and thrombocytes.The process of morphogenesis in all kinds of blood cells except thrombocytes included primitive, native and mature-three stages.The largest proportion of peripheral blood was red blood cells, which shared over 97.56%.Thrombocytes had a high percentage of 52.8% in white blood cells.Neutrophilic granulocytes were the predominant cells among the three granulocytes, accounting for 30.25%. The main haemopoietic tissue of granulocytes and red blood cells were the body kidney and spleen, accounting for 45.18% and 71.62%, respectively.The primitive blood cells were not found in the liver.The development of eosinophils, basophils and neutrophils could be observed simultaneously in the kidney.PAS of white blood cells in each stage showed positive staining, and only granulocytes in every developmental stages showed SBB staining positive.
    • 张波; 王世一; 何昭霞
    • 摘要: 目的 进行急性巨核细胞白血病患者的骨髓细胞形态学、细胞化学和染色体核型分析.方法 选取18例急性巨核细胞白血病患者,进行骨髓采集和分离,获得有核细胞,用以制成普通透射电镜标本、血小板过氧化物酶(PPO)细胞组化电镜标本和骨髓涂片,通过细胞电镜的观察结果和PPO阳性反应结果等对患者的骨髓细胞进行细胞形态学、细胞化学和染色体核型分析.结果 15例患者的骨髓巨核细胞以原始巨核细胞(Ⅰ期)为主,观察结果显示胞间含有少量致密颗粒和α颗粒,胞内不存在分界膜和表面连接管道系统,核较圆,细胞质中存在少量的微管、致密颗粒和膜性囊腔;另外3例患者的骨髓细胞除含有原始巨核细胞外还有大量的幼稚巨核细胞(Ⅱ期)及巨核细胞(Ⅲ期),并且细胞的大小明显不均等;PPO阳性反应的检验结果均呈阳性,阳性率的范围为1%~75%;骨髓涂片观察和染色反应结果显示,原始巨核细胞和幼稚巨核细胞均清晰可见,CD41免疫组化的染色反应呈阳性,并且多为复杂染色体核型.结论 急性巨核细胞白血病患者除少数患者的骨髓细胞包含大量的幼稚巨核细胞和巨核细胞外,大多数患者的骨髓细胞以原始巨核细胞为主,另外患者的PPO阳性反应的阳性率无规律分布、染色体核型复杂.%Objective To analyze the morphology, cytochemistry and chromosome karyotype of bone marrow cells in patients with acute megakaryocytic leukemia. Methods 18 patients with acute megakaryocytic leukemia were enrolled in this study. Nucleated cells were obtained by collecting and isolating the bone marrow of patients, which were used to make ordinary transmission electron microscopy specimens, platelet peroxidase cell histomorphometric specimens and bone marrow smears. Then cell morphology, cytochemistry and chromosome karyotype analysis of bone marrow cells were analysed through the results of cell electron microscopy observation and PPO positive reaction. Results Bone marrow megakaryocytes in 15 patients were predominantly primitive megakaryocytes, and there was a small number of dense particles and α particles between the cells, but without decomposition film and surface connected pipeline system. The bone marrow cells of another 3 patients, with unequal size, not only contained the original megakaryocytes but also had a large number of immature megakaryocytes and megakaryocytes. PPO positive test results were positive and the positive rate in the range of 1% to 75%. Bone marrow smear observation and staining results showed that the original megakaryocytes and immature megakaryocytes are clearly visible, CD41 immunohistochemical staining was positive, and mostly complex chromosome karyotype. Conclusion On the one hand, the main bone marrow cells of the most patients were primitive megakaryocytes and a small number of patients with a large number of immature megakaryocytes and megakaryocytes. On the other hand, the distribution of PPO positive rate was irregular and the chromosome karyotype was complex.
    • 戴锡玲; 任媛媛; 曹建国; 王全喜
    • 摘要: The ultrastructure of the sporoderm and the dynamic of polysaccharides and lipid droplets during the sporangium development in the fern Osmunda japonica Thunb.were studied using light microscopy,transmission electron microscopy and cytochemical techniques.The results showed that:(1) the sporangium of O.japonica consists of a layer of sporangium wall cells,2 layers of tapetal cells and archesporial tissue;(2) the sporoderm consists of exospore and perispore.The exospore,which can be divided into the outer exospore and inner exospore,is thick obviously.But,the perispore is thin and discontinuous.The ornamentations is short baculate and formed by the exospore.The inner exospore is composed of polysaccharides.Both outer exospore and perispore contain lipid materials.(3) Some lipid droplets are seen in the archesporial cells.As the sporangia develop,the amyloplasts in the spore wall cell degenerate.The size of the amyloplasts become small and the number of the amyloplasts decrease gradually.Then these amyloplasts are transported to the plasmodium of the tapetal layer.The amyloplasts are probably transformed into the precursors of sporopollenin and sporopollenin.The sporopollenin are converted into spherical bodies,which pass through the inner membrane of the plasmodium and enter to the sporangium cavity.Finally,these sporopollenin bodies are deposited on the surface of the spores.(4) during spore development,the polysaccharide materials are converted into lipid materials,which are stored in the spores in the form of lipid droplets.The metabolic characteristics of polysaccharides and lipid droplets are observed during the sporangium development,which provided fundamental data and information for studying sporogenesis.%采用光镜、透射电镜和细胞化学技术,对紫萁孢子囊发育过程中孢壁的超微结构和孢子囊内多糖和脂滴的分布及其动态变化进行研究,以探讨紫萁孢子囊发育过程中多糖和脂滴的代谢特征,为蕨类孢子发生的研究提供基础资料.结果表明:(1)紫萁孢子囊由1层囊壁细胞、2层绒毡层和产孢组织构成.(2)紫萁孢子壁由发达而分2层的外壁(外壁内层和外壁外层)和薄的不连续的周壁构成,由外壁形成棒状纹饰的轮廓;孢子外壁内层由多糖类物质构成,外壁外层和周壁均含有脂类物质.(3)在紫萁孢原细胞中观察到少量脂滴;随着紫萁孢壁的形成,囊壁细胞中淀粉粒的大小逐渐变小、数目先增加后减少,它们转运到内层绒毡层原生质团并转化为孢粉素前体物质,再穿过原生质团内膜表面进入囊腔,成为孢粉素团块或以小球形式填加到孢子表面形成孢壁.(4)紫萁孢子囊将多糖类营养物质转化为脂类,以脂滴的形式储藏在孢子中.
    • 摘要: 我们的身体就像一座最奇妙的细胞化学工厂,但多数人不知道自己的身体工厂都做些什么。甚至。还常常“虐待”自己的细胞而不自知……我们身体里的细胞。肉眼是看不到的。如果你在显微镜下看人体细胞的生老病死,你就会了解细胞的奥妙。人在高兴时,细胞很圆润,就像十八岁的年轻人朝气蓬勃,人生气时,细胞就像八十岁的老头,皱皱瘪瘪的,而且。好细胞和生病的细胞完全不一样,癌细胞就是扭扭曲曲,乱七八糟的样子。所以,学会“尊重细胞”。了解细胞需要什么,不需要什么,我们就会避免很多疾病。
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