首页> 外文会议>Conference on Optical Technologies in Biophysics and Medicine V and Saratov Fall Meeting; 20031007-20031010; Saratov; RU >Effect of serotonin on the expression of antigens and DNA levels in Yersinia pestis cells with different plasmid content
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Effect of serotonin on the expression of antigens and DNA levels in Yersinia pestis cells with different plasmid content

机译:血清素对不同质粒含量的鼠疫耶尔森氏菌抗原表达和DNA水平的影响

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Using flow cytometry (FCM) the influence of exogenous serotonin on culture growth, DNA content and fluorescence intensity of cells binding FITC-labelled plague polyclonal immunoglobulins was studied in Yersinia pestis EV (pFra~+, pCad~+, pPst~+), Yersinia pestis KM218 (pFra~-, pCad~-, pPst~-), Yersinia pestis KM 216 (pFra~-, pCad~-, pPst~+). The results have been obtained by FCM showed serotonin accelerated Yersinia pestis EV (pFra~+, pCad~+, pPst~+), Yersinia pestis KM218 (pFra~-, pCad~-, pPst~-) culture growth during cultivation in Hottinger broth pH 7.2 at 28℃ at concentration of 10~(-5) M. The presence of 10~(-5) M serotonin in nutrient broth could modulate DNA content in 37℃ growing population of plague microbe independently of their plasmid content. Serotonin have been an impact on the distribution pattern of the cells according to their phenotypical characteristics, which was reflected in the levels of population heterogeneity in the intensity of specific immunofluorescence determined by FCM.
机译:使用流式细胞仪(FCM)在鼠疫耶尔森菌EV(pFra〜+,pCad〜+,pPst〜+),耶尔森氏菌中研究了外源血清素对培养生长,结合FITC标记的鼠疫多克隆免疫球蛋白的细胞DNA含量和荧光强度的影响鼠疫杆菌KM218(pFra〜-,pCad〜-,pPst〜-),鼠疫耶尔森菌KM 216(pFra〜-,pCad〜-,pPst〜+)。通过FCM获得的结果显示,在Hottinger肉汤中培养期间,血清素加速了鼠疫耶尔森菌EV(pFra〜+,pCad〜+,pPst〜+),鼠疫耶尔森氏菌KM218(pFra〜-,pCad〜-,pPst〜-)培养的生长。在28℃,10〜(-5)M浓度下的pH值为7.2。营养肉汤中存在10〜(-5)M的血清素可以调节37℃生长的鼠疫微生物种群中的DNA含量,而与质粒含量无关。根据细胞表型特征,5-羟色胺已经影响了细胞的分布模式,这反映在通过FCM确定的特异性免疫荧光强度的群体异质性水平上。

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