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BioMEMS for high-throughput handling and microinjection of embryos

机译：BioMEMS用于高通量处理和显微注射胚胎

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Technologies for handling, sorting, and positioning of embryos are increasingly important in biomedicine. In this paper the status for ongoing projects aimed at developing instrumentation for high-throughput treatment of embryos is reviewed. Techniques for positioning of Drosophila (fruit-fly) embryos in 2-D arrays for use in microinjection experiments are especially focused. A method based on fluidic micro assembly is discussed, and important parameters such as immobilization yield, the number of misplaced embryos, and adhesion force of the embryos are reported. A model for the assembly process is described, and simulation results are in good agreement with adhesion force measurements. A fully automated MEMS based system for fruit-fly embryo injection has recently been demonstrated at Stanford University. The first experiments with double-stranded RNA injection proved successful, and the expected genetic modification of the embryos was observed.
机译：在生物医学中，处理，分类和定位胚胎的技术越来越重要。在本文中，综述了正在进行中的旨在开发用于高通量处理胚胎的仪器的项目的现状。特别关注将果蝇（果蝇）胚胎定位在二维阵列中以供显微注射实验的技术。讨论了一种基于流体微装配的方法，并报道了重要参数，例如固定化产率，错位胚胎的数量和胚胎的粘附力。描述了用于组装过程的模型，并且仿真结果与粘附力测量值非常吻合。斯坦福大学最近展示了一种用于果蝇胚胎注射的基于MEMS的全自动系统。首次使用双链RNA注射的实验证明是成功的，并且观察到了预期的胚胎遗传修饰。

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