目的:探讨益生菌FGM发酵黄芪多糖对脑损伤模型小鼠免疫功能的影响.方法:益生菌FGM菌株发酵黄芪药材,所得发酵黄芪粉采用水提醇沉法提取发酵黄芪多糖,发酵黄芪多糖高、中、低浓度给药组小鼠在造模前7天每天分别腹腔注射剂量为200、100、50 mg/kg的发酵黄芪多糖,于末次给药1h后采用线栓法建立小鼠脑损伤模型.称重法测定小鼠脾脏指数、胸腺指数,采用MMT法评价脾淋巴细胞增殖情况.采用酶联免疫吸附法(ELISA)检验炎性因子肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)的表达水平.结果:与模型组比较,发酵黄芪多糖高浓度给药组中脾脏指数、胸腺指数显著升高(P＜0.05);脾淋巴细胞增殖能力显著升高(P＜0.05);TNF-α和IL-1β的含量显著下降(P＜0.05).结论:益生菌FGM发酵黄芪多糖对脑损伤小鼠具有免疫保护作用.%Objective:To investigate the immunologic effect of probiotic FGM fermented astragalus membranaceus on brain damage mice.Methods:Astragalus membranaceus was fermented by probiotic FGM,and ethanol subsiding method was used to extract fermented astragalus polysaccharide in fermented astragalus powder.Fermented astragalus polysaccharide 200,100,50 mg/(kg · d) were intraperitoneal injected daily for 7 consecutive days.The brain damage mice model was established at 1 h after the last dose via suture ligation.Spleen index,thymus index were determined by gravimetric method.The proliferation of splenic lymphocytes was determined by MMT method.Enzyme linked immunosorbent assay(ELISA) was used to determine tumor necrosis factor α(TNF-α) and interleukin 1β(IL-1β).Results:Compared with model group,the spleen index and thymus index was significantly increased in fermented astragalus polysaccharide high concentration administration group (P ＜ 0.05).Splenic lymphocyte proliferation was significantly increased (P＜0.05).The content of TNF-α and IL-1 β decreased significantly(P＜0.05).Conclusion:Probiotic FGM fermented astragalus polysaccharide has immune protective effect on brain damage mice.
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