为分离药用植物中内生真菌，从次级代谢产物中筛选对单胺氧化酶和乙酰胆碱酯酶有较高抑制率的提取物。用分块法分离5种植物，马铃薯葡萄糖琼脂（PDA ）培养基纯化内生真菌，液体培养基发酵，用乙酸乙酯萃取菌液，甲醇提取菌丝体，用酶标法检测提取物对单胺氧化酶和乙酰胆碱酯酶的抑制率。共分离得到45株菌，制备了90种提取物，其中5株菌的菌液提取物对单胺氧化酶有不同程度的抑制率，露蕊乌头39（L RW T‐39）的菌液提取物对单胺氧化酶的抑制率最高，所有提取物对乙酰胆碱酯酶的抑制率不高。表明药用植物内生真菌中存在抗单胺氧化酶的活性成分，值得进一步研究。%Endophytic fungi is isolated from medicinal plants and higher inhibition rate of inhibition of monoamine oxidase(MAO) and acetylcholinesterase(AChE) are found from their secondary metabolites . Endophytic fungi is isolated by issue‐culture method and purified by potato dextrose agar medium . The fermentation of endophytic fungi are extracted by ethyl acetate , and the mycelium of endophytes are extracted by methanol to obtain methanol fractions . All fungi are tested for AChE and MAO inhibiting activity by colorimetric method in 96‐well microplates . Results show that 45 strains of endophytic fungi are isolated and extracted to obtain 90 extracts . Among them ,5 strains of Fungi exhibite significant anti‐monoamine oxidase activity . The ethyl acetate extract of LRWT‐39 are demonstrated to have most high activity against MAO . The active constituents in the endophytic Fungi of medicinal plants are worthy to be further studied .
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