研究了从土壤中筛选产脂肪酶的菌株，利用紫外线、快中子、快中子和磁场复合、γ射线、γ射线和磁场复合诱变，以酶活为筛子进行诱变育种。结果，出发菌酶活较低的一株得到了一株酶活为396.22U/mL的诱变株，此酶活比出发菌株高92倍，并发现此菌对紫外线和快中子比较敏感；而出发菌酶活较高的一株得到了酶活为424.60U/mL发酵液的诱变株，此酶活为出发菌株3.0倍。在此基础上，初步探讨了快中子、γ射线及磁场复合处理在产脂肪酶菌种诱变中的作用，并认为，在产脂肪酶菌株的诱变中快中子诱变更为有效。%Four methods of breeding high producing strain of Lipase are studied. As a result, the Lipase activity produced by one strain is 93 times as high as befor, and the Lipase activity produced by another is 3.0 times. On these bases, the effects of four methods in breeding are discussed, and the usinng Fast Neutron is more effective method in breeding.
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