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首页> 外文期刊>Bioprocess and Biosystems Engineering >Pellet-dispersion strategy to simplify the seed cultivation of Aspergillus niger and optimize citric acid production
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Pellet-dispersion strategy to simplify the seed cultivation of Aspergillus niger and optimize citric acid production

机译：颗粒分散策略可简化黑曲霉的种子培养并优化柠檬酸生产

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摘要

Citric acid (CA) as an extremely important platform compound has attracted intense attention due to wide applications and huge markets. Here, we proposed a novel method, using pellet inoculation to replace spores, and constructed the seed recycling cultivation process, effectively avoided the longtime (spore preparation 30 days) of seed culture (including spores germination 12 h) in the traditional batch-fermentation. On this basis, using pellet-dispersion strategy, the bottleneck caused by the mycelium structure was overcome, with the seed restoring high cell-viability with CA titer (11.0 g/L) even in the eighth batch compared to that in the control (4.6 g/L). The optimum morphology of these recycling cultured seeds for CA production was dispersed pattern rather than pellets. And the CA production was 130.5 g/L on average in 5 L five-conjoined-fermenters recycling eight batches, especially increasing 3.1 g/L compared with the control. To our knowledge, this is the first that reported the application of these strategies in effective production of CA. Our fermentation strategies not only significantly enhanced CA productivity, but also severed as a promising stepping-stone for other fermentations dominated with the filamentous fungi.
机译：柠檬酸（CA）作为极为重要的平台化合物，由于其广泛的应用和巨大的市场而引起了广泛的关注。在这里，我们提出了一种新的方法，即通过颗粒接种代替孢子，并构建了种子循环培养过程，有效避免了传统分批发酵中种子培养的长时间（孢子准备30天）（包括孢子萌发12h）。在此基础上，采用颗粒分散策略，克服了由菌丝体结构引起的瓶颈，即使与对照（4.6）相比，第八批的CA滴度（11.0 g / L）也能使种子恢复高细胞活力。 g / L）。这些用于CA生产的循环培养种子的最佳形态是分散模式而不是颗粒。回收八批5L五联发酵罐中CA的平均产量为130.5 g / L，与对照相比，增加了3.1 g / L。据我们所知，这是第一个报道了这些策略在有效生产CA中的应用的报告。我们的发酵策略不仅显着提高了CA的生产率，而且被切断为丝状真菌主导的其他发酵的有希望的垫脚石。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2017年第1期|45-53|共9页
  • 作者

    Wang Baoshi; Chen Jian; Li Hua; Sun Fuxin; Li Youran; Shi Guiyang;

  • 作者单位

    Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China|Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China|Jiangsu Guoxin Union Energy Co Ltd, Wuxi 214203, Peoples R China;

    Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China;

    Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China;

    Jiangsu Guoxin Union Energy Co Ltd, Wuxi 214203, Peoples R China;

    Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China|Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China;

    Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China|Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Citric acid; Aspergillus niger; Pellet dispersion; Recycling cultivation; Seed morphology;

    机译：柠檬酸;黑曲霉;颗粒分散;循环培养;种子形态;

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