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首页> 外文期刊>Bulletin of the National Research Centre >Genetic Improvement of Trichoderma reesei for Cellulase Enzymes Production Through Mutation Breeding
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Genetic Improvement of Trichoderma reesei for Cellulase Enzymes Production Through Mutation Breeding

机译：里氏木霉通过突变育种生产纤维素酶的遗传改良

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摘要

The present study aimed to investigate the possibility of inducing genetic variability in the fungus Trichoderma reesei which produces cellulases upon treatment with different mutagensis.The fungal strain NRRL - 12368 was treated with UV-light, NTG, and combined treatment with both of them or combined treatment with UV-light and NaNO_2. The results indicated that the treatment with UV-light induced 183 colonies, 48 of them are morphological variants and 6 are auxotrophic mutants. When the NTG mutagen was applied, 48 colonies were isolated, 20 of them are morphological variants and only one is auxotrophic mutant. Using the combination between UV and NTG, 64 colonies were obtained 24 of them are morphological variants and only one is auxotrophic. The combination between UV and NaNO_2 gave 49 colonies, 11 of them are morphological variants and one is auxtrophic mutant. These mutants were evaluated for their productivity concerning the three cellulase enzymes FPase, CMCase and 3-glucosidase. Some mutants overyielded the parental strain in the cellulases production. The mutant S26 produced 180%, 102% and 31.6% FPase, CMCase and 3-glucosidase more than the original strain, respectively. No relationship can be found between the requirements of auxotrophic mutants and their cellulase activities, since some mutants lost their enzymes activity, some others produced one or more of these enzymes, whereas two mutants produced the three enzymes more than their parental strain.
机译：本研究的目的是探讨在不同的穆塔格犬处理后产生纤维素酶的里氏木霉属真菌诱导遗传变异的可能性。对真菌菌株NRRL-12368进行紫外线，NTG处理，并同时使用它们或联合使用紫外线和NaNO_2处理。结果表明，用紫外线诱导的183个菌落处理，其中48个是形态变异，6个是营养缺陷型突变体。当使用NTG诱变剂时，分离出48个菌落，其中20个是形态变异，只有一个是营养缺陷型突变体。使用UV和NTG的组合，获得了64个菌落，其中24个是形态变异，只有一个是营养缺陷型。 UV和NaNO_2的结合产生了49个菌落，其中11个是形态变异，一个是营养缺陷型突变体。对这些突变体的生产力进行了评估，涉及三种纤维素酶FPase，CMCase和3-葡糖苷酶。一些突变体在纤维素酶生产中使亲本菌株超产。突变体S26产生的FPase，CMCase和3-葡萄糖苷酶分别比原始菌株多180％，102％和31.6％。营养缺陷型突变体的需求与其纤维素酶活性之间没有关系，因为一些突变体丧失了其酶活性，另一些突变体产生了一种或多种这些酶，而两个突变体产生的三种酶比其亲本菌株多。

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