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首页> 外文期刊>British Biotechnology Journal >Production of Some Polysaccharides fromKojic Acid Producing Aspergillus oryzae var.effusus NRC14 Biomass
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Production of Some Polysaccharides fromKojic Acid Producing Aspergillus oryzae var.effusus NRC14 Biomass

机译：从曲酸生产米曲霉NRC14生物质生产一些多糖

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Aims: The present investigation describes the utilization of Aspergillus oryzae var. effusus NRC14 biomass producing kojic acid in the production of fine chemicals that have valuable applications such as chitin, chitosan, β-1, 3 glucan and /or their derivatives.Methodology: Experiments of cell growth and substrate utilization were conducted in static cultures with identified medium composition. The technology involved in the extraction of their biopolymers is quite simple and the size scale is compatible with those currently used. This work was performed in Department of Microbial Chemistry, NRC.Results and Conclusion: The utilization of A. oryzae var. effusus NRC14 biomass waste actually will cover a part of the cost production of kojic acid; therefore, clean cell walls preparations were isolated from the fungus biomass at different stages during kojic acid fermentation in a glucose salts medium. The major chitin constituent was extracted from the prepared cell walls from six days cultured A. oryzae var. effusus NRC14 biomass by cold conc. HCl or by 5% of LiCl/ DMAA solvent in yields 4.78% and 2.44%, respectively. Among the chitin family, chitosan has received special attention since it has several applications. Chitosan was extracted from alkali insoluble material (cell wall) obtained from 12 days cultured biomass by using different concentrations of acetic acid (0.5-2.5%) the results showed that the highest yield of chitosan was 4.65% at a concentration of 2% acetic acid. The isolated product was characterized by infrared spectrum. Chitosan- glucan complex was isolated in 17.94% yield by treating the A. oryzae var. effusus NRC14 biomass produced from kojic acid production for 12 days with 40% aqueous NaOH for 6h. at 95°C. Chitosan-glucan complex was fractionated into its components. The amount of extracted chitosan was 3.5%; the residue obtained after solubilization of chitosan by acid treatment was characterized as being mostly insoluble glucan (94%). Chitin-glucan complex was isolated from 6 days cultured A. oryzae var. effusus NRC14 biomass in yield 17.034%. The lyophilized product was fractionated into its components (chitin and glucan) in yields 16.36% chitin, 82.72% β, 1-3 glucan. The infrared spectra of the isolated products as well as of standard samples proved the identity of the isolated materials as chitosan, chitin and glucan.
机译：目的：本研究描述了米曲霉变种的利用。方法：在静态培养物中进行细胞生长和底物利用实验，确定中等成分。提取生物聚合物的技术非常简单，其规模与目前使用的技术兼容。结果和结论：米曲霉的利用。 NRC14生物质废液实际上将弥补曲酸成本生产的一部分;因此，在曲酸在葡萄糖盐培养基中发酵的不同阶段，从真菌生物质中分离出了干净的细胞壁制剂。从六天培养的米曲霉的制备的细胞壁中提取主要几丁质成分。通过冷浓缩处理出NRC14生物量。 HCl或5％的LiCl / DMAA溶剂的产率分别为4.78％和2.44％。在几丁质家族中，壳聚糖由于具有多种用途而备受关注。用不同浓度的乙酸（0.5-2.5％）从培养12天的生物质中得到的碱不溶性物质（细胞壁）中提取壳聚糖，结果表明，当浓度为2％时，壳聚糖的最高收率为4.65％。 ％乙酸。分离的产物通过红外光谱表征。通过处理米曲霉变种分离壳聚糖-葡聚糖复合物，收率为17.94％。曲酸生产中产生的effusus NRC14生物质，用40％的NaOH水溶液生产12天，持续6h。在95°C下。将壳聚糖-葡聚糖复合物分级分离为其组分。壳聚糖的提取量为3.5％。通过酸处理将壳聚糖溶解后获得的残余物的特征在于大部分是不溶的葡聚糖（94％）。从培养的米曲霉6天中分离出几丁质-葡聚糖复合物。 NRC14生物质的产量为17.034％。将冻干的产物分馏成其组分（几丁质和葡聚糖），产率为16.36％的几丁质，82.72％的β，1-3个葡聚糖。分离出的产物以及标准样品的红外光谱证明了分离出的物质为壳聚糖，几丁质和葡聚糖的身份。

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