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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Sequence Variation in the porA Gene of a Clone of Neisseria meningitidis during Epidemic Spread
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Sequence Variation in the porA Gene of a Clone of Neisseria meningitidis during Epidemic Spread

机译：流行期间脑膜炎奈瑟氏球菌克隆porA基因的序列变异

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The ET-15 clone within the electrophoretic type (ET)-37 complex ofNeisseria meningitidis was first detected in Canada in 1986 and has since been associated with outbreaks of meningococcal disease in many parts of the world. While the majority of the strains of the ET-37 complex are serosubtype P1.5,2, serosubtype determination of ET-15 strains may often be incomplete, with either only one or none of the two variable regions (VRs) of the serosubtype PorA outer membrane protein reacting with monoclonal antibodies. DNA sequence analysis of the porA gene from ET-15 strains with one or both unidentified serosubtype determinants was undertaken to identify the genetic basis of the lack of reaction with the monoclonal antibodies. Fourteen different porA alleles were identified among 38 ET-15 strains from various geographic origins. The sequences corresponding to subtypes P1.5a,10d, P1.5,2, P1.5,10d, P1.5a,10k, and P1.5a,10a were identified in 18, 11, 2, 2, and 1 isolate, respectively. Of the remaining four strains, which all were nonserosubtypeable, two had a stop codon within the VR1 and the VR2, respectively, while in the other two the porA gene was interrupted by the insertion element, IS1301. Of the strains with P1.5,2 sequence, one had a stop codon between the VR1 and VR2, one had a four-amino-acid deletion outside the VR2, and another showed no expression of PorA on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Our results reveal that numerous genetic events have occurred in theporA gene of the ET-15 clone in the short time of its epidemic spread. The magnitude of microevolutionary mechanisms available in meningococci and the remarkable genetic flexibility of these bacteria need to be considered in relation to PorA vaccine development.
机译：1986年在加拿大首次发现脑膜炎奈瑟氏球菌电泳型（ET）-37复合体中的ET-15克隆，此后与世界许多地方的脑膜炎球菌疾病爆发有关。虽然大多数ET-37复合物的菌株是血清亚型P1.5,2，但对ET-15菌株的血清亚型测定通常可能是不完整的，血清亚型PorA的两个可变区（VR）中只有一个或一个都不存在外膜蛋白与单克隆抗体反应。对带有一个或两个未确定的血清亚型决定簇的ET-15菌株 porA 基因进行DNA序列分析，以确定缺乏与单克隆抗体反应的遗传基础。在来自不同地理来源的38株ET-15菌株中鉴定出14个不同的 porA 等位基因。在18、11、2、2和1个分离物中鉴定出了与亚型P1.5a，10d，P1.5、2，P1.5、10d，P1.5a，10k和P1.5a，10a相对应的序列，分别。在其余所有四个菌株中，它们都是不可血清亚型的，其中两个在VR1和VR2中分别具有终止密码子，而在另外两个中， porA 基因被插入元件IS 1301 。在具有P1.5，2序列的菌株中，一个在VR1和VR2之间具有终止密码子，一个在VR2外部具有四个氨基酸缺失，另一个在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上未显示PorA表达。 。我们的结果表明，ET-15克隆的 porA 基因在流行期间很短时间内发生了许多遗传事件。脑膜炎球菌中可利用的微进化机制的数量以及这些细菌的显着遗传灵活性需要与PorA疫苗的开发相关。

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