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首页> 外文期刊>African Journal of Biotechnology >Fermentative conversion of raw glycerol into 1,3-propanediol by isolated Klebsiella pneumoniae 141B stain: Optimization of culture variables
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Fermentative conversion of raw glycerol into 1,3-propanediol by isolated Klebsiella pneumoniae 141B stain: Optimization of culture variables

机译：分离的肺炎克雷伯氏菌141B染色剂将原始甘油发酵转化为1,3-丙二醇：培养变量的优化

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摘要

In this study, 1,3-propanediol (1,3-PDO) production by?Klebsiella pneumoniae?141B strain using raw glycerol as substrate was investigated. Taguchi L18 orthogonal array (OA) was adopted to optimize nutritional (raw glycerol, yeast extract and calcium carbonate), physiological (incubation temperature and medium pH) and microbial (inoculum level) fermentation variables. These controlling fermentation factors were selected based on one variable at a time methodology. Raw glycerol, temperature and calcium carbonate were the most significant factors among the selected factors followed by pH and inoculum level. Yeast extract, however, showed the least significance on 1,3-PDO production at individual level, while in combination, it elucidated a remarkable severity index values of 64.81 and 58.17% with pH and temperature, respectively. At optimized environment, 53% contribution towards 1,3-PDO production was observed from selected fermentation parameters. The model was experimentally validated, yielding a 1,3-PDO production of 0.62 mol mol-1?of raw glycerol, which represented a 19% increase when compared to the non-optimized medium.
机译：在这项研究中，研究了以粗甘油为底物的肺炎克雷伯菌肺炎克雷伯氏菌141B菌株生产的1,3-丙二醇（1,3-PDO）。采用Taguchi L18正交阵列（OA）优化营养（原始甘油，酵母提取物和碳酸钙），生理（培养温度和中等pH）和微生物（菌种水平）发酵变量。这些控制发酵因子是基于一次方法中的一个变量进行选择的。原始甘油，温度和碳酸钙是所选因素中最重要的因素，其次是pH和接种量。然而，酵母提取物在单个水平上对1,3-PDO产生的影响最小，而结合起来，其在pH和温度下的严重度指数分别为64.81和58.17％。在最佳环境下，从选定的发酵参数中观察到对1,3-PDO产生的贡献为53％。该模型已通过实验验证，可产生0.62 mol mol-1？的甘油的1,3-PDO生成，与未优化的培养基相比，增加了19％。

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